derivation of escherichia coli o157h7 from its o55h7 precursor推导的大肠杆菌o157h7 o55h7前体.pdfVIP

Derivation of Escherichia coli O157:H7 from Its O55:H7 Precursor 1,2 1 1,2 3 4 1 1,2 5 Zhemin Zhou , Xiaomin Li , Bin Liu , Lothar Beutin , Jianguo Xu , Yan Ren , Lu Feng , Ruiting Lan , Peter R. Reeves6, Lei Wang1,2,7* 1Tianjin Economic-Technological Development Area School of Biological Sciences and Biotechnology, Nankai University, Tianjin, China, 2 Tianjin Research Center for Functional Genomics and Biochip, Tianjin, China, 3 National Reference Laboratory for Escherichia coli, Federal Institute for Risk Assessment, Berlin, Germany, 4 State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Beijing, China, 5 School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia, 6 School of Molecular and Microbial Biosciences, University of Sydney, Sydney, Australia, 7 Tianjin Key Laboratory of Microbial Functional Genomics, Nankai University, Tianjin, China Abstract There are 29 E. coli genome sequences available, mostly related to studies of species diversity or mode of pathogenicity, including two genomes of the well-known O157:H7 clone. However, there have been no genome studies of closely related clones aimed at exposing the details of evolutionary change. Here we sequenced the genome of an O55:H7 strain, closely related to the major pathogenic O157:H7 clone, with published genome sequences, and undertook comparative genomic and proteomic analysis. We were able to allocate most differences between the genomes to individual mutations, recombination events, or lateral gene transfer events, in specific lineages. Major differences inclu