diminished thrombogenic responses by deletion of the podocalyxin gene in mouse megakaryocytes减少形成血栓的反应通过删除podocalyxin基因在小鼠巨核细胞.pdfVIP

Diminished Thrombogenic Responses by Deletion of the Podocalyxin Gene in Mouse Megakaryocytes 2. ´ 1. 3 ´ ´ 1,2 ´ Miguel Pericacho , Sonia Alonso-Martın , Susana Larrucea , Consuelo Gonzalez-Manchon , Darıo ´ 2 ´ ´ 1 1,2 1,2 Fernandez , Ines Sanchez , Matilde S. Ayuso , Roberto Parrilla * ´ 1 Department of Cellular and Molecular Medicine, Centro de Investigaciones Biologicas (CSIC), Madrid, Spain, 2 CIBER de Enfermedades Raras (CIBERER), Madrid, Spain, ´ 3 Laboratorio de Inmunologıa, Hospital de Cruces, Baracaldo, Spain Abstract Podocalyxin (Podxl) is a type I membrane sialoprotein of the CD34 family, originally described in the epithelial glomerular cells of the kidney (podocytes) in which it plays an important function. Podxl can also be found in megakaryocytes and platelets among other extrarenal places. The surface exposure of Podxl upon platelet activation suggested it could play some physiological role. To elucidate the function of Podxl in platelets, we generated mice with restricted ablation of the podxl gene in megakaryocytes using the Cre-LoxP gene targeting methodology. Mice with Podxl-null megakaryocytes did not show any apparent phenotypical change and their rates of growth, life span and fertility did not differ from the floxed controls. However, Podxl-null mice showed prolonged bleeding time and decreased