role of oxidative stress in transformation induced by metal mixture角色转换氧化应激诱导的金属混合物.pdfVIP

Hindawi Publishing Corporation Oxidative Medicine and Cellular Longevity Volume 2011, Article ID 935160, 11 pages doi:10.1155/2011/935160 Research Article Role of Oxidative Stress in Transformation Induced by Metal Mixture ´ Silva-Aguilar Martın, Rojas Emilio, and Valverde Mahara Departamento de Medicina Genomica y Toxicologıa Ambiental, Instituto de Investigaciones Biomedicas, ´ ´ ´ Universidad Nacional Autonoma de Mexico, Ciudad Universitaria 04510, DF, Mexico ´ ´ Correspondence should be addressed to Valverde Mahara, mahara@biomedicas.unam.mx Received 8 August 2011; Accepted 7 September 2011 Academic Editor: Guillermo Zalba ´ Copyright © 2011 Silva-Aguilar Martın et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Metals are ubiquitous pollutants present as mixtures. In particular, mixture of arsenic-cadmium-lead is among the leading toxic agents detected in the environment. These metals have carcinogenic and cell-transforming potential. In this study, we used a two step cell transformation model, to determine the role of oxidative stress in transformation induced by a mixture of arsenic- cadmium-lead. Oxidative damage and antioxidant response were determined. Metal mixture treatment induces the increase of damage markers and the antioxidant response. Loss of cell viability and increased transforming potential were observed during the promotion phase. This findi