silicon-based technology for ligand-receptor molecular identification硅基技术中的分子识别.pdfVIP

Hindawi Publishing Corporation Journal of Atomic, Molecular, and Optical Physics Volume 2012, Article ID 948390, 5 pages doi:10.1155/2012/948390 Research Article Silicon-Based Technology for Ligand-Receptor Molecular Identification Annalisa Lamberti,1 Carmen Sanges,1 Nunzia Migliaccio,1 Luca De Stefano,2 Ilaria Rea,2 Emanuele Orabona,2 Giuseppe Scala,3 Ivo Rendina,2 and Paolo Arcari1, 4 1 Department of Biochemistry and Medical Biotechnologies, University of Naples “Federico II”, 80131 Naples, Italy 2 Institute for Microelectronics and Microsystems, Unit of Naples, National Council of Research, 80131 Naples, Italy 3 Department of Clinical and Experimental Medicine, University of “Magna Graecia”, 88100 Catanzaro, Italy 4 CEINGE Advanced Biotechnology s.c.a.r.l, 80145 Naples, Italy Correspondence should be addressed to Paolo Arcari, arcari@unina.it Received 23 June 2011; Accepted 4 October 2011 Academic Editor: Ali Hussain Reshak Copyright © 2012 Annalisa Lamberti et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. One of the most important goals in the fields of biology and medicine is the possibility to dispose of efficient tools for the characterization of the extraordinary complexity of ligand-receptor interactions. To approach this theme, we explored the use of crystalline silicon (cSi) technology for the realization of a biotechnological device in which the ligand-receptor interactions are revealed by means of optical measurements. Here, we describe a chemical procedure for the functionalization of microwell etched on silicon wafers, and the subsequent anchoring of bi