biolog微生物鉴定步骤（biolog微生物鉴定步骤）.docVIP

biolog微生物鉴定步骤（biolog微生物鉴定步骤） Collected by oneself Mistakes are unavoidable For reference only In case of error Please correct me! Thank you Biolog microbiological identification procedure Detection principle The Biolog microbial identification system tests the ability of microorganisms to utilize or oxidize substances in the identification board Testing produces characteristic purple hole patterns Metabolic fingerprinting All essential nutrients and biochemical reagents are added 96 Kong Banzhong in advance Four tetrazolium is a redox dye Indicates the utilization of carbon sources The identification procedure is very simple The isolated strains were expanded and cultured Then make the inoculation solution and add it to the identification board In the process of training Some of the chemicals in the pores are oxidized and give colour to purple Control holes (A-1) and negative holes are still colorless The metabolic pattern can be formed by culturing the identification board for 4-6 hours or 16-24 hours under the corresponding culture conditions System software automation and database comparison If you can find the right match An identification result can be obtained Two required equipment and consumables: ? medium, inoculation fluid, sodium thioglycolate, long cotton, inoculation rod, storage tank, eight pipettes, pipette head, turbidity meter, turbidity standard, temperature control box and the identification of the corresponding training board The inoculation solution is prepared by itself Inoculation rod, liquid storage tank can be used instead of domestic brands Three identification procedure: Biolog microbiological identification sample handling steps Isolation and purification medium BUG+B General medium sheep BUA+B Anaerobic medium sheep BUY Yeast culture medium 2%ME 2% wort extract Gram staining and morphological observation of colony forming bacteria Gram staining result Gram-negative Gram-positive Anaerobe Yeast filamentous fungi Confirmation test Positive