C反应蛋白基因启动子T757C多态性及不稳定性心绞痛关系.docVIP

C反应蛋白基因启动子T757C多态性及不稳定性心绞痛关系 　 作者：戚猛， 何国平， 高磊， 沈丹丹， 金鸣锋， 张寄南 【摘要】 目的： 探讨中国苏皖地区汉族人群C反应蛋白（Creaction protein, CRP）基因启动子T757C多态性与不稳定性心绞痛（unstable angina pectoris, UAP）的可能关系。方法： 采用病例对照研究方法，以165例UAP 患者（病例组）和282例非冠心病者(对照组)作为研究对象，应用聚合酶链反应-限制性片段长度多态性（polymerase chain reactionrestriction fragment length polymorphism，PCRRFLP）方法检测CRP基因T757C多态性。结果： 与对照组相比，UAP组的TT，TC和CC基因型频率比较均无统计学意义（P值均gt;0.05）。 T等位基因频率在UAP组和对照组分别为77.6%和79.1%，两组间差异无统计学意义（Pgt;0.05）。多元Logistic回归分析显示CRP基因T757C多态性与UAP的发生无显著性关联（Pgt;0.05）。 结论： 在中国苏皖地区汉族人群中，CRP基因启动子T757C多态性与UAP无显著相关性。 【关键词】 C反应蛋白； 基因多态性； 不稳定性心绞痛 ［Abstract］ Objective: To investigate the possible association between Creaction protein(CRP) gene T757C polymorphism in the promoter region and unstable angina pectoris(UAP) in a Chinese Han population of Suwan region. Methods： This study was conducted with a casecontrol design including 165 patients with UAP(UAP group) and 282 control subjects who were free from coronary artery disease(control group). The T757C polymorphism in CRP gene was determined by polymerase chain reaction and restriction fragment length polymorphism analysis. Results： Compared with the control group, the frequencies of TT，TC and CC genotype were not significantly different in UAP group（Pgt;0.05）. The prevalence of T allele was 77.6% and 79.1%(Pgt;0.05) respectively in the UAP group and control group. Multivariate logistic regression analysis indicated that there was no significant correlation between CRP gene T757C polymorphism and UAP（Pgt;0.05）. Conclusion： Our findings suggest that the T757C polymorphism in CRP gene may be not the risk for UAP among the Chinese Han population of Suwan region. ［Key words］ Creaction protein； gene polymorphism； unstable angina pectoris 不稳定性心绞痛（unstable angina pectoris, UAP）是冠状动脉内不稳定的粥样斑块继发病理改变，使局部心肌血流量明显下降。不稳定粥样斑块的肩部是最常发生破裂的部位，而此处C反应蛋白（Creaction protein, CRP）沉积较多［1］。CRP主要由白介素6通过剂量依赖方式刺激肝细胞及活化巨噬细胞合成，可促进血管内皮细胞增生、迁移、动脉内膜增厚；具有调节单核细胞聚集作用, 与膜攻击复合物共同存在于早期动脉粥样硬化病变内，造成血管内膜损伤