络气郁滞证大鼠动物模型建立及评价.docVIP

络气郁滞证大鼠动物模型建立及评价 　 作者：吴相春，来静，吴以岭，贾振华，王洪涛，张秋艳 【摘要】 目的建立络气郁滞证大鼠动物模型并进行评价。方法对大鼠慢性束缚6周建立络气郁滞证模型，观察模型大鼠的精神、饮食量、体质量、蔗糖水摄取量，血清皮质酮(CORT)、血浆内皮素(ET)，血清5羟色胺(5-HT)、NO以及主动脉组织内皮细胞超微结构的变化。结果络气郁滞证模型表现为精神萎靡，反应迟钝，摄食量减少，体质量增长缓慢，蔗糖水摄取量、摄食量及体重均明显低于对照组(Plt;0.05或Plt;0.01);模型组大鼠血浆ET升高、血清CORT升高，5-HT、NO降低(Plt;0.05或Plt;0.01);内皮细胞超微结构发生明显改变。结论利用慢性束缚法6周，通过观测大鼠体质量、摄食量、蔗糖水摄取量、CORT、ET、NO、5-HT的变化，初步建立络气郁滞证大鼠动物模型，制作方法简单，评价指标客观，为脉络血管系统病的研究提供良好的实验基础。 【关键词】 络气郁滞证; 动物模型 　　Abstract：ObjectiveTo establish and evaluate the rat animal model with collateral qi stasis. MethodsTo establish animal model with collateral qi stasis in 6 weeks by chronic constraint. To observe the changes of consciousness, appetite, body weight, the intake of cane sugar water, and the levels of CORT, ET, NO, 5-HT. The ultramicrostructure change of aorta was observed by transmission electron microscope. ResultsThe animal model rats showed deprementia, dull,no appetite，increasing slow of body weight，the cane sugar water intake was lowered significantly (Plt;0.01 or Plt;0.05), the levels of CORT,HCY,ET were increased significantly(Plt;0.01 or Plt;0.05)，and the levels of 5-HT,NO were lowered significantly (Plt;0.01 or Plt;0.05). The ultramicrostructure of aorta changed obviously.ConclusionThe animal model with collateral qi stasis is established with chronic constraint. The method has been succeed through observation the changes of body weight, cane sugar water intake, appetite, CORT，ET，NO，5-HT, and ultramicrostructure of aorta. The method is easy and the evaluated indexes are objective, which provide the good experiment foundation for the study of vessel-collateral and vascular system disorders. 　　Key words：Stasis of collateral qi; Animal model 　　络气郁滞、络气虚滞引起的络脉自稳状态功能异常与血管内皮功能障碍具有内在一致性，均为“脉络-血管系统病”的始动因素并贯穿病变全过程，成为运用络病理论研究血管病变的切入点[1]。高同型半胱氨酸血症是内皮功能障碍的独立危险因素，而应激是引起高同型半胱氨酸血症的主要原因。我们以慢性束缚法建立络气郁滞证大鼠动物模型，进行络气郁滞证候的研究。 　　1 材料与仪器 　　1.1 动物及分组健康雄性Wistar大鼠20只，体质量200～230 g，购于北京维通利华实验动物中心，大鼠5只/笼，饲养在河北以岭医药研究院药理实验室，按体重随机分为两组，即正常对照组和络气郁滞模型组。正常饲料饮食。 　　1