virological and serological findings in rousettus aegyptiacus experimentally inoculated with vero cells-adapted hogan strain of marburg virus病毒学和血清学结果rousettus aegyptiacus实验接种州立cells-adapted霍根的马尔堡病毒株.pdfVIP

Virological and Serological Findings in Rousettus aegyptiacus Experimentally Inoculated with Vero Cells- Adapted Hogan Strain of Marburg Virus 1,2 1 3 1 Janusz T. Paweska *, Petrus Jansen van Vuren , Justin Masumu , Patricia A. Leman , 1 1 4 4 1 Antoinette A. Grobbelaar , Monica Birkhead , Sarah Clift , Robert Swanepoel , Alan Kemp 1 Center for Emerging and Zoonotic Diseases, National Institute for Communicable Diseases of the National Health Laboratory Service, Sandringham, South Africa, 2 Division Virology and Communicable Disease Surveillance, School of Pathology, University of the Witwatersrand, Johannesburg, South Africa, 3 Southern African Center for Infectious Disease Surveillance, Morogoro, Tanzania, 4 University of Pretoria, Pretoria, South Africa Abstract The Egyptian fruit bat, Rousettus aegyptiacus, is currently regarded as a potential reservoir host for Marburg virus (MARV). However, the modes of transmission, the level of viral replication, tissue tropism and viral shedding pattern remains to be described. Captive-bred R. aegyptiacus, including adult males, females and pups were exposed to MARV by different inoculation routes. Blood, tissues, feces and urine from 9 bats inoculated by combination of nasal and oral routes were all negative for the virus and ELISA IgG antibody could not be demonstrated for up to 21 days post inoculation (p.i.). In 21 bats inoculated by a combination of intraperitoneal/subcutaneous route, viremia and the presence of MARV in different tissues was detected on days 2–9 p.i., and IgG antibody on days 9–21 p.i. In 3 bats inoculated subcutaneously, vi