对赤芍醇提浸膏及水提液治疗胆汁淤积型肝损伤药效筛选.docVIP

对赤芍醇提浸膏及水提液治疗胆汁淤积型肝损伤药效筛选 　 作者：罗琳，姚敏，韩萍，窦志华，徐济良 【摘要】 目的：筛选有治疗胆汁淤积型肝损伤作用的赤芍提取物。方法：将小鼠随机分成正常对照组、模型组、赤芍醇提浸膏高、中、低剂量组，水提液高、中、低剂量组和阳性药赤丹退黄颗粒组；用α－萘异硫氰酸酯建立胆汁淤积型小鼠模型，连续给药6天后，检测血清ALT、BIL、ALP、TBA等指标，并进行病理组织观察和半定量分析。结果：赤芍醇提浸膏能显著降低血清DBIL、ALP、ALT和AST值（Plt;0.05或Plt;0.01），并能明显改善α－萘异硫氰酸酯引起的组织损伤（Plt;0.01），中剂量作用与阳性对照药相似；而水提液没有治疗作用（Pgt;0.05）。结论：赤芍治疗胆汁淤积型肝炎的有效药物成分主要在赤芍醇提浸膏中。 【关键词】 肝损伤；胆汁淤积；赤芍；α-萘异硫氰酸酯；小鼠 [Abstract] Objective: To screen the effective extract of Radix Paeoniae Rubra on cholestatic hepatic injury. Methods: All mice were randomly divided into nine groups : the control group, the model group, the extracta sicca groups of high dose, medium dose and low dose, the water extract groups of high dose, medium dose and low dose, and the Chidan granula group. The cholestatic hepatic injury animal models were made by alpha-naphthylisothiocyanate（ANIT）. All animals were given the drugs or normal saline for six days. The serum indexes of ALT, BIL, ALP, TBA were tested and the pathological changes of the livers were analyzed by semiquantitative method. Results: The extracta sicca decreased the levels of DBIL, ALP, ALT and AST in serum (Plt;0.05 or Plt;0.01), and it also significantly improved the liver injury induced by ANIT (Plt;0.01). The extracta sicca group of medium dose had the same effect as the Chidan granula group.There was no effect in the water extract groups (Pgt;0.05). Conclusion: The pharmacodynamic ingredients healing cholestatic hepatic injury are in the extracta sicca of Radix Paeoniae Rubra. [Keywords] Hepatic injury; Cholestasis; Radix Paeoniae Rubra; Alpha-naphthylisothiocyanate; Mouse 赤芍为毛茛科植物芍药（Paeonia lactiflom Pall）或川赤芍（Paeonia veitchii Lynch）干燥根。其味苦，微寒，归肝经，具有清热凉血，散瘀止痛之功能[1]。大量的临床和实验研究表明，赤芍治疗黄疸型肝炎疗效确切[2，3]，但迄今为止对其研究仅停留在单味药或复方层次，而其治疗黄疸型肝炎的药效物质成分并不清楚。 赤芍总苷是赤芍的主要活性成分，但其是否为赤芍治疗黄疸型肝炎的活性成分尚有待研究证实[4]。鉴于此，本课题先对赤芍的水提部位和醇提部位进行退黄降酶药效的筛选，为进一步提取分离确切的药效部位奠定基础。 1 材料与方法 1.1 实验动物 健康ICR小鼠，雌雄各半，体重20±2 g，由南通大学动物实验中心提供, 动物使用许可证：SYXK(苏)2007-0021。 1.2 药物 （1）α－萘异硫氰酸酯（alpha-napht