动脉粥样硬化兔主动脉平滑肌细胞钙激活钾通道活性变化及前列腺素E-%2c1-对其影响的研究.pdf

1.0±0.15 kilogram. (2)Rabbits from the AS group were fed with high fat fodder(0.5%cholesterin + 5%lard + 15%yolk power + 79.5%ordinary food), then the cholesterin in food was removed after three weeks. Ordinary food was fed to rabbits in the Control group. Both groups’ rabbits had been fed for eight weeks. (3)Single aortic smooth muscle cell was obtained by an enzymatical treatment. Patch clamp technique was used to record the single KCa channel current. The signal of channel open probability(Po), mean open dwell-time(To), mean close dwell-time(Tc) and current amplitude were recorded by the software of Pclamp 7.0. After adding different concentration of Ca2+(10-8,5×10-8, 10-7, 5×10-7, 10-6M) and PGE (10-9, 5×10-9, 10-8, 5×10-8, 1 -7 10 M) to bath solution respectively, the above parameters were observed again. Results ：(1)KCa channel activity: in the cell-attached and inside-outside patch, KCa channels had significant voltage dependence in the Control group and the AS group. Compared with the Control group, the activity in the AS group was higher. In symmetrical 140mM k+ solutions, at membrane potential of +40mV, using the cell-attached patch, Po was 0.0316 ± 0.0205 in the Control group and 0.2401 ± 0.1215 in the AS group with statistic difference(p0.05)(n=10), Tc was 238.752 ± 203.253ms and 19.861 ± 12.524ms(p0.001)(n=10), conduction was 205.650 ± 37.125pS and 195.225 ± 42.825pS(p ＞0.05)(n=10), and there was no statistical difference in To and Am. Using the inside outside patch, Po was 0.0183 ± 0.0085 in the Control group and 01652 ± 0.0764 in the AS group with statistic