高糖干预骨髓来源mapc细胞周期的研究.pdfVIP

19 卷 3 期 生命科学研究 灾燥造援员9 晕燥援3 圆园15 年6 月 蕴蚤枣藻 杂糟蚤藻灶糟藻 砸藻泽藻葬则糟澡 Jun. 圆园15 高糖干预骨髓来源MAPC 细胞周期的研究 罗 旻, 刘泽灏, 李 纯 ， （中南大学 湘雅医院 内分泌科 中国湖南 长沙410078 ） 摘 要 骨髓来源MPAC 是具有多向分化潜能的成体干细胞袁高糖是干扰MAPC 细胞分化趋势的重要因素遥通 过流式细胞技术分析高糖对MAPC 细胞周期的影响遥研究显示,高糖迟滞MAPC 细胞的细胞周期在G 1/S 期遥进 一步研究的结果提示袁高糖可以通过TGF-茁1 调控ERK1/2 信号通路,选择性上调P21 蛋白表达,抑制MAPC 的 细胞周期进程袁此时的MAPC 细胞Oct4 高表达袁具有多项分化能力渊处于非分化状态冤遥 关键词 高糖曰MAPC 细胞曰细胞周期曰TGF-茁1曰P21 曰ERK1/2 中图分类号 R587.1 文献标识码 A 文章编号 1007-7847(2015)03-0229-08 Regulation of Cell Cycle of MAPCs via High-glucose LUO Min, LIU Ze-hao, LI Chun (Endocrinology Dep artment, X iangya Hosp ital, Central South Univ ers ity,Changsha 4 10078, H unan, China) Abstract ：Multipotent adult progenitor cells (MAPCs) derived from bone marrow stem cell ，with multi-direc tional differentiation. High glucose is one of the important interferential factors for the differentiation of MAPCs. FACS was performed for analysis of cell cycle of MAPCs incubated in high-glucose（HG ）. The data indicated that cell cycle of MAPCs was arrested in G1/S. The mechanism indicated that cell cycle of MAPCs was arrested via high glucose -induced expression of TGF -茁1, with up -regulation expression of P21 via ERK1/2 signaling. At the same time, high -expression level of Oct4 in MAPCs exhibit extensive expansion capability and are able to differentiate at the single cell level into functional multiple cell lineages of all three germinal layers（un-differentiation ）. Key words: high glucose ；multipotent adult progenitor cells (MAPCs) ；cell cycle ；transforming grows factor- 茁1（TGF