HPLC测定槐花药材不同部位芦丁与槲皮素含量.docVIP

HPLC测定槐花药材不同部位芦丁与槲皮素含量 　　摘要：目的 比较槐花不同部位（槐花、槐米及枝梗）中芦丁和槲皮素的含量。方法 采用高效液相色谱法进行测定，Ultimate XB-C18色谱柱（4.6 mm×250 mm，0.45 ?m），水-甲醇为流动相梯度洗脱，流速1 mL/min，柱温35 ℃，检测波长256 nm，进样量20 ?L。结果 芦丁含量为槐米槐花枝梗；槲皮素含量整体较低，且槐花槐米枝梗。结论 槐花各部位芦丁含量较高，且含量差别不大。槐花中的槲皮素含量明显高于槐米及其枝梗。 关键词：槐花；不同部位；芦丁；槲皮素；含量比较 DOI：10.3969/j.issn.1005-5304.2012.12.016 中图分类号：R284.1 文献标识码：A 文章编号：1005-5304（2012）12-0043-03 Content Determination of Rutin and Quercetin in Different Parts of Sophora Japonica L. Medicinal Materials by HPLC DU Yu-ran1， HE Fu-yuan1，2，3， ZHOU Yi-qun1， XIE Xiang-gui1， SHI Ji-lian1，2 （1.Department of Pharmaceutics， Hunan University of Chinese Medicine， Changsha 410208， China；2.Property and Pharmacodynamic Key Laboratory of Chinese Matria Medica， State Administration of Chinese Medicine， Hunan University of Chinese Medicine， Changsha 410208， China；3.Pharmaceutical Preparation Technology and Evaluation Laboratory of Chinese Matria Medica， Hunan University of Chinese Medicine， Changsha 410208， China） Abstract：Objective To compare rutin and quercetin content of different parts （flower， bud and pedicel） of Sophora Japonica L.. Methods HPLC method was used with Ultimate XB-C18 column （4.6 mm×250 mm， 0.45 ?m）， water and methanol as solvent system with gradient elution. The flow rate was 1 mL/min， column temperature was 35 ℃， detection wavelength was set at 256 nm， and injection volume was 20 ?L. Results The content of rutin was budflowerpedicel. The content of quercetin was low on the whole， and in sequence of flowerbudpedicel. Conclusion In the different parts of Sophora Japonica L.， rutin content is high with little difference. Quercetin content of flower is significantly higher than in bud and pedicel. Key words：Sophora japonica；different parts；rutin；quercetin；comparison of content 槐花为豆科植物槐Sophora japonica L.的干燥花及花蕾，前者称“槐花”，后者称“槐米”，具有凉血止血、清肝泻火的功效[1]。现代研究表明，其黄酮类主要成分芦丁和槲皮素均具有多种药理作用[2]。近年来，随着医药工业的发展及保健品的开发，槐花作为黄酮类的提取原料，其需求量逐年递增。但因栽种局限性、生长半野生化及环保限制采收等原因使槐花产量减少[3]。市售槐花药材中除了槐花和槐米外，还含有大量的枝梗，在炮