生物反应器课件 bioreactor design Select cell culture method.ppt

Bioreactor design Select cell culture method Content Introduction Cell culture specification Agitation Temperature control Sterilization CIP Bioreactor design considerations Goals: Max growth or max productivity Need stringently maintained process conditions Agitation (max transfer) Homogenous condition Bioreactor design considerations Repeatable transfer of energy, substrates, metabolites. - Good mixing depends on: nature medium rheology of the broth airflow rate/ type of aeration kind and number of impellers/baffles organism behaviour (shearstress) geometry of vessel (H/D) accurate speed control Bioreactor design considerations Bioreactor design considerations for cell culture Top driven agitator Marine impellers Tipspeed up to 1 m/s No baffles Micro porous sparger Gas flows up to 0.1 vvm Headspace aeration Aspect ratio = 1 at working volume CIP-able design Bioreactor design considerations Shape ASME Bottoms Hemispherical Bottoms Jacket Design Conventional Dimpled Half Pipe Ports Tri-Clamp, DN, NA-connect, Ingold Type, Flange type Bioreactor design considerations ASME Head(s) Hemispherical Head(s) Agitation Seals Mechanical Face Seals Lip Seals Magnetic Drives Impellers Turbine Marine Pitched Blade Others Agitation Agitation - turbine impeller For unaerated water @ 20 C in a fully baffled tank the following formula applies: P= 4.5 x 10-13 * Di5 * N3 * F Di = Impeller Diameter in Inch N = RPM of Shaft F = One (1) for one impeller (1.8) for two impellers (2.4) for three impellers Agitation - turbine impeller Tip Speed should also be considered because of tendency to shear cells. Do not exceed 1 m/s for cell culture And 5 - 6 m/s for microbial cultures. Temperature control Temperature control Cooling System: remove 50 to 100 Watts/Liter of Volume. Heating Systems: One (1) degree C per minute between 25 and 45 C Spargers Ring Type Single Orifice Sintered Filters Membrane (0.2/0.45 micron pore size) Usually made of