PEG-6000干旱胁迫对5种牧草种子萌发的影响研究.PDFVIP

中国农学通报 2015,31(26):7-11 Chinese Agricultural Science Bulletin PEG-6000干旱胁迫对5种牧草种子萌发的影响研究 陈小倩，徐庆国 （湖南农业大学农学院，长沙410128 ） 摘 要：为了解5 种冷季型牧草种子萌发期的抗旱性，采用聚乙二醇(PEG-6000)模拟干旱，分别以5% 、 10% 、15%聚乙二醇溶液作渗透介质，研究了不同干旱胁迫对紫花苜蓿、白三叶、多年生黑麦草、红三叶 和细弱翦股颖等5 种牧草种子的发芽率、发芽势、发芽指数、活力指数等4 个指标的影响。研究结果表 明：随PEG-6000 浓度的增加，白三叶、紫花苜蓿、多年生黑麦草、细弱翦股颖的发芽率、发芽势、发芽指数 和活力指数都呈现下降趋势；红三叶在5%的水分胁迫下发芽率、发芽势、发芽指数和活力指数均高于对 照。经回归统计检验和系统聚类分析综合评价结果，该5 种牧草种子抗旱性强弱依次为：细弱翦股颖 红三叶 紫花苜蓿 白三叶 多年生黑麦草。 关键词：PEG-6000 ；牧草；种子；萌发期；干旱胁迫；抗旱性 中图分类号：S54 文献标志码：A 论文编号：cas EffectsofPEG-6000DroughtStressonSeedGerminationofFiveForageGrassSpecies ChenXiaoqian,XuQingguo (CollegeofAgriculture,HunanAgriculturalUniversity,Changsha410128) Abstract:Inordertounderstandtheseeddroughtresistanceoffivecool-seasonforagegrassspeciesduring seedgerminationperiod,PEG-6000 attheconcentrationof 5%, 10%and 15%wereusedasthepenetration mediatosimulatedroughtstress.Andeffectsofdifferentdroughtstresses ongerminationofdifferentcool- seasonforagegrassseeds(Medicagosativa L., Trifoliumrepens L., Loliumperenne L., Trifoliumpratense, Agrostis tenuis Sibth.) were studied. Four indicators which were germination rate, germination potential, germination index and seed vitality index were measured. The results showed that germination rate, germinationpotential,germinationindexandseedvitalityindexof T.repens,M.sativa ,L.perenne ,A.tenuis alldecreasedwiththeincreaseofPEG-6000concentration.Thefourindicatorsof T. pratense increasedat5% PEG-6000 concentration.Regressionstatisticaltestsandhierachicalclusteranalysisshowedthatdrought resistanceofthefiveforagegrassspecies wasinanorderas A.tenuisT.pratenseM.sativaT. repensL. perenne. Keywords:PEG-6000;foragegrass;seeds;germinationp