pEGFP-N1-hTERT真核表达载体的构建与表达鉴定.pdfVIP

ofAnhui 安衡农业科学。．burnal Asri．Sei．2008．36{35)：15370—15372。15478责任编辑张蕾责任校对吴晓燕 pEGFP-N1-hTERT真核表达载体的构建与表达鉴定 科技大学国家干细胞工程技术研究中心陕西分中心，陕西杨凌712100；3．宁夏职业技术学院，宁夏银川750002) 棱细胞中表达。[结论]该研究为建立大鼠永生化NSCs系奠定了基础。 关键词GFP；hTERT；真核表达栽体；构建；鉴定 中图分类号S188文献标识码A 文章编号晒17—6611(2006)35—15370一∞ The of Vector ConstructionandIdentificationEukar-yoticExpression pEGFP-NI-h，rERT of jnWestern LI elal of ofEducationforProtectionandUtilization Resources Cbina， Yong (KeyLaboratoryMinistry SpecialBido画cal NingxiaUniversity，Yinehuan，Ningxia750021) Abstract aimofthis istoconstruct vector andobserveits ineu- f Objective]Thestudy eukaryotieexpressionpEGFP．NI．h7rERT expression vector wasconstructedwith and karyoticcells．fMethod]TheeukaryoticexpressionpEGFP—NI-hTERT pcl-neo—hTERTpEGFP—N1plasmids。 se- and山e ofhumantelomerasereverse Wasconfirmeddouble andDNA accuracy transcriptase(hTERT)geRefragment by enzymedigestion the vectorsintoratfetalneuralstem ceUs(NSCs)。the quencinganalysis．AftertransfectingpEGFP-NI-hTERTeukaryotieexpression protein ofhumantelomerasereverBe were observed fluorescentjntheeels．andthecorrect- 】ocalization transcriptaseindirecdy throughgreen protein flessof RT．PCRandWesternBlot structureof constructed w釉certificated pEGFP，NI．hTERT by analysis．[Result]111epEGFP—Nl，h1ERT vectorwas andit in ConclusionThis iaidafound