同时针对VEGF和p53腺病毒干扰载体的构建及干扰作用.pdfVIP

中国肿瘤生物治疗杂志2005 … 。。…’ ’… n J CancerBiother ·189· [文章编号]1007-385X(2005)03-0189-04 同时针对VEGF和p53腺病毒干扰载体的构建及干扰作用 褚衍六1，赵幼安1，李文通2，郭成浩2，宋现让3，迟伟玲4，魏玲3(1．山东大学齐鲁医院消化科，济南 4．山东大学医学院分子生物学教研室，济南250012) [摘要] 作用。方法：先分别构建能干扰p53和VEGF的质粒载体，依次将构建干扰载体的干扰序列和H1启动子序列切下并连接到 中的VEGF和p53 细胞中VEGF和p53mRNA的表达。 [关键词]RNA干扰；腺病毒；基因治疗；血管内皮生长因子；p53 [中图分类号]R730．54 [文献标示码]A ConstructionofRNAiAdenovirus both andVEGF Targetingp53 Synchro- andItsGene Function nously Silencing CHU Yan．1iu7，ZHAOYou—an7，LIWen—ton92，GUOCheng—ha02，SONGXian．rang’，CHIWei—lin94，WEI Ling’(_，．DepartmentofGastroenterology，QiluHospitaU，Shandong partmentofPathology，MedicalSchool，ShandongUniversity，Ji’nan ofCancer Research Tumor and Institute，ji’nan250117，China；4．DepartmentofBiochemis— Center，ShandongHospital andMolecular 250012，China) try Biology，MedicalSchool，ShandongUnivenity，Ji’Tlan constructRNAiadenovirusbothVEGFand inthesame Objective：To targeting p53 [Abstract] theH1 andRNAi VEGFand wereconstructed sequence interferingplasmidstargeting p53 respectively．Thenpromoter werecutand to to wastransfeetedintoBJ5183cells successively pAdTraek／VEGF／p53，which ligatedpAdTrack generate infec‘ to after theadenovirusafter containing generatepAd／VEGF／p53homologousrecombination，harvesting pAdEasy一1 real—timeafter