犬瘟热病毒H基因原核表达质粒的构建.pdfVIP

贵州农业科学2011，39(9)：174～176 Sciences GuizhouAgricuhural 犬瘟热病毒H基因原核表达质粒的构建 周莉1，刘志杰1，曾智勇1’2。，汤德元1，李春燕1，王彬1，张晓杰1，甘振磊1，王凤1 (1．贵州大学动物科学学院，贵州贵阳550025；2．贵州省动物疫病研究室，贵州贵阳550025) [摘 的预防奠定基础，以已构建含有犬瘟热病毒H基因的pMDl8一H质粒为模板，利用合成的特异性引物进行 824 PCR扩增，得到l H基因原核表达质粒。 [关键词]犬瘟热病毒；H基因；原核表达；构建 [中图分类号]$852．655 [文献标识码]A Constructionof OfH Vector ProkaryoticExpression Geneof Canine Virus Distemper ZHOULil，LIUZhi．iiel，ZENG Zhi—yon91孙，TANGDe-yuanl，LIChun—yanl， WANG Binl，ZHANG Zhen—leil，WANG Xiao-jiel，GAN Fen91 AnimalScience。Guizhou 550025；2．Guizhoa (1．Collegeof University，Guiyang，GuizhouLaboratory Animal 550025，China) {or EpidemicDisease，Guiyang．Guizhou Abstract：ToofferaccordancewithH as CDV proteinexpressiondiagnosisantigen，detectspecific and thebasisCDV onthe for constructed antibodyprovide prevention，based pMDl8～Hplasmidcontaining canine virusN frameofl824was chain distemper gene．theopenreading bp amplifiedbypolymerase one of and withthesame reaction(PCR)usingpairprimers．Hgene pET32a(+)weredigested procedure， thenH wasclonedinto recombinant wasidentified gene pET32a(+)．Thepositive plasmidpET32a(+)一H andthe resultsshowedthatH wasinthecorrectinserted and frame。 sequencing