药物发现技术.pptVIP

药物发现技术Techniques applied in the drug discovery ;药物发现的技术概况;药物发现技术主要类型;高通量药物筛选主要研究内容;1、药物靶点（Drug targets）研究;基因功能筛选程序;Figure 3. Molecular targets of drug therapy. Classification according to biochemical criteria. Based on a modern standard work of pharmacology, the molecular targets of all known drugs that have been characterized as safe and effective have been collected and listed according to their biochemical nature (62). Jürgen Drews. Drug Discovery: A Historical Perspective. Science 2000; 287 (5640): 1960-1964 ;已知药物靶点基本特点;药物靶点的基本特点;药物靶点的基本特点;药物靶点研究的基本过程;药物靶点研究的基本过程;2、HTS筛选技术;2、HTS筛选技术;HTS筛选技术; ; ;To determine the concentration of FITC-SR-1;To determine the time of incubation and stability of the signal;Displacement assay;Evaluating the FP-based HTS:Z’ value: 0.75; ;3、生物芯片技术; Surfaces modified with polysaccharide film;Figure 1. Fluorescence images of opioid R-μ(A)and androgen R-LBD (B) microspots after incubation with solutions containing naloxone-FITC and FluormoneTM AL Green respectively at the concentration of 1 nM in the absence (i) and presence (ii) of 3% BSA for blocking nonspecific absorption.; BSA Lox-1 R;;;;;4、HCS筛选技术;HTS筛选技术;HCS筛选技术;5.模型评价;Evaluation parameter;6. 先导化合物评价(Evaluation of lead compounds); 先导化合物评价(Evaluation of lead compounds); 先导化合物评价(Evaluation of lead compounds);先导化合物评价(Evaluation of lead compounds);先导化合物评价(Evaluation of lead compounds);先导化合物评价(Evaluation of lead compounds);先导化合物评价(Evaluation of lead compounds);7. 筛选策略研究(Strategy for drug discovery);筛选策略研究(Strategy for drug discovery);筛选策略研究(Strategy for drug discovery);筛选策略研究(Strategy for drug discovery);筛选策略研究(Strategy for drug discovery);筛选策略研究(Strategy for drug discovery);筛选策略研究(Strategy for drug discovery);筛选策略研究(Strategy for drug discovery);筛选策略研究(Strategy for drug discovery);筛选策略研究(Strategy for drug discovery);基因功能筛选、功能??究与药物发现 from Gene to Drug;筛选策略研究(Strategy for drug discovery);谢谢