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APaseActivityinSoybeanandCommonBean
asRelatedtoPAvailability
TianJiang
SouthChinaAgriculturalUniversity,Guangzhou510642,China
ABSTRACT
Soybean(Glyciuemax伍.)Merr.)andcommonbean(Phaseolus
vulgarisL.)aretworepresentativegrainlegumes,whosenutritional
valueisuniversallyrecognizedsincetheycontainhighlevelsof
proteinandahighproportionofoilandcarbohydrates.Grainlegumes
alsoplayaveryimportantroleinsustainableagriculturedevelopment
fortheirabiliyttofertilizethesoilbybiologicalnitrogenfixation.
However,theproductionofsoybeanandcommonbeanissignificantly
limitedbylowphosphorus(P)availabilityinthesoil,especiallyinthe
tropicsandsubtropics.Plantshaveevolvedavarietyofstrategiesto
adapttolowPavailability.Amongthepossiblemechanisms,acid
phosphatases(APases)arebelievedtobeimportantformany
physiologicalprocessesrelatedtoPefficiencyinplants.Butstudies
aboutthephysiologicalfunctionsofAPasearelimitedandtheresults
areconflicting.Inthepresentstudy,systematicfieldnadlaboratory
experimentswereconductedtoinvestigatethephysiologicaland
biochemicalcharacteristicsofAPasesindifferenttissues/organsas
relatedtoPavailabilityinordertogetabetterinsightintotheir
physiologicalfunctions.Anappliedcorecollectionofsoybean
germplasmwasusedinafieldexperimenttostudythecorrelation
betweenleafAPaseactivityandPefficiency.LeafAPasepropertiesas
relatedtoPavailabilitywerefurthercharacterizedinanutrient
solutionculturewith8soybeangenotypesselectedfromthefieldstudy.
Subsequently,APasecharacteristicsintherootsandcoytledonswere
studiedintwosoybeangenotypesandtwocommonbeangenotypes
contrastinginPefficiencyand/orleafAPaseactivity.Finally,oneof
theAPaseisoformswaspurifiedrfomtheleavesofacommonbean
genotype,anditsbiochemicalandphysiologicalpropertieswere
studied.Ourresultsshowedthat:
1.TherewasagreatgenotypicvariationforleafAPaseactivity
amongthetestedsoybeangenotypesint
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