高效液相色谱法测定大鼠血浆中柚皮素浓度研究.pdfVIP

高效液相色谱法测定大鼠血浆中柚皮素浓度 陈 孝 邓乐民 (中山大学附属第一医院药学部 广州 510080) [论文摘要] 目的 柚皮素 Naringenin富集于西柚汁和番茄中 也可以由柚皮苷 Naringin水解生成 它具 有抗雌激素 降脂 抗氧化等活性作用 但同时也是一种对细胞色素 P450具有很强抑制作用的活性 物质 能与某些药物发生相互作用 从而导致药物不良反应的发生 本研究的目的在于建立一套可靠 稳定 灵敏的测定Naringenin血浆浓度的方法 以期用于摄入柚皮素或柚皮苷后的药物代谢动力学 和药物相互作用研究 方法 分析柱为HYPERSIL BDS C250 mm4.6 mm5 m 流动相为乙腈 18 pH 2.5磷酸缓冲液(3367)流速1.0 mL/min检测波长为288 nmNaringenin和370 nm 柱温为室温 结果 柚皮素血浆浓度在 0.0510 g/ml范围内 浓度与峰面积比有良好的线性关系 r 0.9998平均方法回收率为 105.65.8 ,日内RSD3.8 日间RSD4.5 结论 本 方法简单 可靠 准确 可用于测定柚皮素血浆浓度 关键词 柚皮苷 柚皮素 高效液相色谱法 Chen Xiao Deng Lemin (Department of Pharmacy, The first Affiliated Hospital, Sun yat-sen University. Guangzhou 510080) [Abstract] Objective The flava none naringenin exhibits estrogenic, cholesterol- lowering and antioxidative properties. Meanwhile it can alter the pharmacokinetics of a variety of clinically used drugs resulting in drug interactions. This study was designed to establish a precise, accurate and sufficiently sensitive method to measure the plasma concentration of naringenin. Method A HYPERSIL BDS C18 column 250 mm×4.6 mm 5ìm was adopted and the temperature was room temperature. Mobile phase was a mixture of acetonitrile and pH 2.5 phosphoric acid buffer 33 67 and flow rate was 1.0 mL/min. The UV detector was operated at 288 nm for naringenin and 370 nm for quercetin. Results Good linear calibration curve was obtained over the range of 0.05ìg/mL to 10ìg/mL in rat plasma (r=0.9998). The recovery rate was 105.6±5.8 and the coefficients of intra-day and inter-day variation did not exceed 3.8 , 4.5 , respectively. 1 Conclusions This method is convenient, stable and accurate, which may