膳食纤维对高脂高胆固醇膳食诱导脂代谢紊乱小鼠实验的研究.docVIP

精品论文 参考文献 膳食纤维对高脂高胆固醇膳食诱导脂代谢紊乱小鼠实验的研究 杨江涛 韩淑芬 焦俊 张薇 秦立强(通讯作者) 　　（苏州大学医学部公共卫生学院 215123） 　　【摘要】 目的：探讨膳食纤维（dietary fiber，DF）对脂代谢紊乱小鼠的作用。方法：48只雄性C57BL/6J小鼠按体重随机分为阴性对照组、模型对照组、燕麦DF组和全小麦DF组，以相应饲料连续喂养24周，测定各组小鼠体重、摄食量、血清总胆固醇、甘油三酯、血糖以及炎性因子肿瘤坏死因子alpha;和白介素-6等指标，同时进行肝脏组织病理学检查。结果：与模型对照组比较，燕麦DF和全小麦DF降低了脂代谢紊乱小鼠的体重、血清TC、FFAs 和TNFalpha;水平以及肝匀浆TG水平，燕麦DF降低了血糖水平。两组干预组小鼠肝脏脂肪变性程度明显减轻。结论：DF对高脂高胆固醇饲料诱导的脂代谢紊乱小鼠的血脂水平及炎性因子水平具有较好的改善作用，燕麦DF对总胆固醇和血糖的作用优与全小麦DF，全小麦DF对甘油三酯的作用优于燕麦DF。 　　【关键词】 膳食纤维；脂代谢紊乱；血脂；炎性因子 　　【中图分类号】R965 【文献标识码】A 【文章编号】2095-1752（2015）20-0051-03 　　Effect of dietary fiber on lipid metabolic disorder mice induced by high-fat/cholesterol diet 　　Yang Jiangtao, Han Shufen, Jiao jun, Zhang Wei, Qin Liqiang. School of public health, Medical College of Soochow University, Jiangsu Province, Suzhou 215123, China 　　【Abstract】Objective To explore the effect of dietary fiber ( DF) on lipid metabolic disorder mice. Method Forty-eight male C57BL/6J mice were randomly divided into four groups according to body weight. Negative control group, model control group, oat fiber group and wheat bran fiber group were given respective experimental diets for 24 weeks. Body weight, food consumption, serum total cholesterol (TC), triglyceride (TG), glucose and TNFalpha;, IL-6 were measured respectively, liver specimens were examined with pathological detection. Results Compared with model control group, both oat fiber group and wheat bran fiber group significantly decreased body weight, serum TC, FFAs ,TNFalpha; and TG level in liver tissue, and oat fiber group decreased glucose level . The two intervention group significantly decrease the degree of hepatic steatosis. Conclusion DF intake could improve lipid and inflammatory level of lipid metabolic disorder mice induced by a high-fat/cholesterol diet. 　　【Key words】Dietary fiber; Lipid metabolic disorder; Llpid level; Inflammatory factor 　　大量流行病学研究已证实，富含膳食纤维（dietary fiber，DF）的食物能有效降低血脂[1]，改善高脂饮食引起的脂代谢紊乱[2]。本研究采用美