肝x受体激动剂对大鼠急性肺损伤中肺泡巨噬细胞吞噬功能的影响word论文.docxVIP

有统计学差异。（4）T组MermRNA的表达水平与蛋白质表达明显高于L组、N组，差异具有统计学意义（P均小于0.05）。结论(1) 肝 X受体激动剂对脂多糖诱导的大鼠急性肺损伤具有明显的保护作用。（2）脂多糖诱导的急性肺损伤时肺泡巨噬细胞的吞噬功能明显降低，肝X受体可明显增强肺泡巨噬细胞的吞噬功能，其同时明显增强肺组织Mer基因表达明显增强。（3）肝X受体对急性肺损伤的保护机制可能是通过增强肺泡巨噬细胞的吞噬功能与上调Mer基因的表达实现的。关键词肝X受体巨噬细胞吞噬功能急性肺损伤Mer2Modulation EffectsofLiverXReceptorsagonistsonalveolar macrophagephagocytosis ofratsduringAcute Lung Injury AbstractObjective:ToobservetheeffectofliverXreceptoragonistsT0901317onthealveolar macrophagephagocytosis ofAcuteLungInjury,andto exploretheeffect ofliverX receptor on acutelunginjuryand its possibleprotectivemechanism.Methods:60maleSDratsweredividedrandomlyintothreegroups:controlgroup (groupN),LPStreatmentgroup(groupL)andT0901317+LPStreatmentgroup(group T). groupTwithliverXreceptoragonistT0901317irrigated1weeklater,thentheratswere killedafterintraperitonealinjectionofLPS6h.groupLandthegroupNindimethyl sulfoxideirrigated1weeklater,respectively,intraperitonealinjectionofLPS,saline,the ratswerekilledafterintraperitonealinjection6h.Andthenweretakentothelungtissue andobservethechangesingeneralandpathologicalmorphologicalchanges.Finally,To collectbronchoalveolarlavagefluidof eachgroup,andtoseparateandpurificateof alveolarmacrophages,MPOstainingdeterminatealveolarmacrophagestheMPOpositive stainingrate,inaddition,usingpurifiedalveolarmacrophagesandthenmeasuredthe phagocytosispercentageofchickenredbloodcellsandphagocyticindex,andthe expressionofMer mRNAandproteininlung tissuewere detectedbyRT-PCRand Western blot techniques.Results:(1)Thegeneralmorphologyoflungtissue:thegroup Noflungtissuehas agood elasticityand thedensityis uniform,smooth Envelope,with pink color; thegroupLoflung tissuewith areableeding,tissue swelling,unevencolor;thegroupToflungtissuewas swell- ling, but thetissueinjuryofgroupTwas lighter thangroupL, no largehemorrhage. (2)Pathologicalchangesoflungtissue:IngroupN, thelungtissue alveolar wall is thin,3clearand completed,no inflammatorycells infiltrated in the alveolarclearance andalveoli- arspaces,no obviousbro