力达霉素对人宫颈癌Caski细胞增殖的影响及其机制.docVIP

力达霉素对人宫颈癌Caski细胞增殖的 影响及其机制 哗麵占景琼 三峡大学肿瘤微环境与免疫治疗湖北省重点实整室 R的探讨力达霉素对人宫颈癌Caski细胞增殖的影响及其可能的机制。方法将 对数生长期的人宫颈癌Caski细胞随机分为低、中、高浓度组及对照组，低、中、 高浓度组均加入含力达霉素的RPM1 1640完全培养基100 PL，分别调整其终浓 度为1、2. 5、5 ng/m L,对照组仅加入等体积RPMI 1640完全培养基。作用24 h, 采用MTT法检测细胞增殖能力（以吸光度值表示），计算细胞生存率;采用流式 细胞术检测细胞周期及线粒体膜电位降低比例;透射电子显微镜不观察细胞形态 学改变，计算细胞凋亡率及裂亡率。结果低、中、高浓度组细胞增殖能力及细 胞生存率均低于对照组，且高浓度组降低更明显（P均＜0.01）。对照组及中浓 度组S期细胞比例均高于低、高浓度组（P均〈0.05）。低、中、高浓度组G0/G1 期细胞比例均低于对照组，G2/M期细胞比例均高于对照组，且中、高浓度组变 化更明显（P均〈0.05）。低、屮、高浓度组及对照组细胞线粒体膜电位降低比 例分别为（34. 77±5. 33） %、 （37. 87±5. 73） %、 （18. 37±3. 91） %、 （4. 17±0. 65） %;低、中、高浓度组细胞线粒体膜电位降低比例均高于对照组，且 低、中浓度组均高于高浓度组（P均＜0.05）。透射电镜下可见对照组细胞形态 正常，低、中、高浓度组存在多种形态的死亡细胞，包括凋亡、坏死、裂亡的细 胞。对照组及高、低、屮浓度组细胞凋亡率及裂亡率均依次升高，两组间比较P 均〈0.05。结论力达霉素可抑制人宫颈癌Caski细胞增殖，并呈浓度依赖性，其 机制可能与诱导细胞凋亡或裂亡有关。 关键词： 宫颈癌;力达霉素;人宫颈癌Caski细胞;细胞凋亡;细胞裂亡;线粒体膜电 位； E-mai1:4554080@qq. 基金：国家自然科学基金资助项目 Effect of lidamycin on proliferation of human cervical cancer Caski cells LU Hua QIN Ye ZHAN Jingqiong WANG Yanlin China Three Gorges University, Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy; Abstract： Objective To investigate the effect of lidamycin on the proliferation of human cervical cancer Caski cells and its underlying molecular mechanisms. Methods Human cervical cancer Caski cells were randomly divided the experimental groups ( including the low-dose, medium-dose and high-dose groups) which were separately treated with 1, 2. 5 and5 ng/m L lidamycin (100 u L) for 24 h, and the control group which was added with the same volume of RPMI-1640. MTT assay was used to detect the inhibition rate of proliferation. The cell cycle and mitochondrial membrane potential of Cas Ki cells were measured by flow cytometry. Transmission electronic microscope was used to observe the morphological changes of Caski cells. We calculated the apoptosis rate and mitotic catastrophe rate. Results The proliferation rate and survival rate of the experimental groups were significantly lower than those of the control group, and the dec