(精选)蛋白、RNA结构与功能 (Protein, RNA Structure and Function)教学课件.pptVIP

D Dobbs ISU - BCB 444/544X: Protein Structure Function Additional Structural Levels Super-secondary elements Motifs Domains Foldons Soon after the discovery of let-7, the Mello, Zamore, and Hannon labs reported that CLICK gene inactivation by RNAi and the control of developmental timing by stRNAs, are interconnected processes that share certain molecular components. -- The most prominent component was the highly-conserved nuclease Dicer, which cleaves double-stranded precursor molecules into stRNAs and siRNAs. Essential background on microRNAs - Family of small non-coding RNAs found in animals and plants Endogenous precursor RNA foldbacks are processed by the enzyme Dicer to mature single-stranded 21 or 22 nt microRNAs RNA interference involves longer, perfect duplex RNA (exogenous or endogenous), processed by Dicer to ~21mer siRNAs Characterized animal microRNAs direct translational inhibition by basepairing to 3’ UTRs of protein coding mRNAs and are often involved in developmental control. Pairing between microRNA and mRNA is always partial/incomplete (usually multiple bulges/loops). There are typically several microRNA complementary sites per regulated mRNA. Plant microRNAs and siRNAs generally have perfect or near-perfect complementarity to mRNAs and can trigger mRNA degradation. * * D Dobbs ISU - BCB 444/544X: Protein Structure Function 11/4/05 Protein Structure Function D Dobbs ISU - BCB 444/544X: Protein Structure Function Announcements Exam 2 - Has been graded - Will be returned at end of class today Grade statistics – 444 Average = 81/100 544 Average = 100/118 Questions? D Dobbs ISU - BCB 444/544X: Protein Structure Function Announcements BCB 544 Projects - Important Dates: Nov 2 Wed noon - Project proposals due to David/Drena Nov 4 Fri PM - Approvals/responses tentative presentation schedule to students Dec 2 Fri noon - Written project reports due Dec 5,7,8,9 class/lab - Or