1、TIMP-2在SD大鼠眼前段组织的表达.docVIP

1、TIMP-2在SD大鼠眼前段组织的表达 眼科新进展2006年4月第26卷第4期RecAdOphthalmolVo1.26No.4April2006?257? 作者简介:潘佳鸿,女.1977年6 月出生,江苏吴江人,硕士.师从 上海复旦大学附属华山医院眼科 主任叶纹教授.联系电话:021—H);E—mail:pjh一7706 @126.tom AboutPANJia.Hong:Female.born inJune.1977.Masterdegree.Tel: +86.21H):E-mail: pjh一7706@126.tom 收稿日期:2005—1l-22 修回日期:2006.O1.O2 本文编辑:董建军 基金项目:上海市科委资助课题 (编号:004119018) 作者单位:加0135上海市,上海 浦东新区公利医院眼科(潘佳 鸿);200040上海市,复旦大学 附属华山医院眼科(叶纹); 200o25上海市.上海交通大学 医学院附属瑞金医院眼科(钟一 声) Receiveddate:Nov22.2005 Accepteddate:Jan2,2006 岫蛐item:Sl_Ⅲ出iSdence andTechnical~lst0eProvideFi— naneialAssistan~Prdect(No: 01M119018) FromtheDepartmentofOphthal— molo~,GongtiH0s两inShanghai PudongArea(PANJ~Hong), Shanthm200135.China:De~rt一 ofOphthalmology.Huashan HospitalAffiliatedFudanUniverd一 (YEWen),Shanghai200040, China;DepartmentofOphthoi— molo~.RuifinH0s砸Affiliated nJio~tongUniversityMedi— ca/CoUeg~(ZHONGYi~ng), Shan~i200025,China MMP.2,TIMP.1,TIMP.2在SD大鼠 眼前段组织的表达△ 潘佳鸿叶纹钟一声 Expressionsofmatrixmetalloproteinase-2and tissueinhibitorofmetalIoproteinase-1,2inthe anteriorsegmenttissuesofnormalrateyes PANJia-Hong.YEWen,ZHONGYi—Sheng 【Keywords】metrixmetalloprotelnase;tissuelnhlbltorsofmetallopro- telnase;rat;trabecularmeshwork;ciliarybody;age-relatedchanges 【Abstract】ObjectiveToinvestigatetheexpressionofmetrlxmetallopro- teinase-2(MMP-2)andtissueinhibitorofmetalloproteinase-1,2(TIMP-1,2)in anterlorsegmenttissuesofnormalrateyesindifferentagesandtodetermine therolesofMMPandTIMPintrabecularoutflowanduveoscleraloutflowin normaleyes.MethodsImmunohlstochemistrywasus~_dtodetectthePres- enceofMMP-2andTIMPl.2inanteriorsegmenttissuesofnormalrateyesin differentages.Theimmunohistochemlcalstainingwasfurtheranalyzedand theintensityofreactionproductsweredetectedwithanimage-processingsys- tem.ResultsMMP_2andTIMP_1,2wereexpressedintheiris,ciliarybody, trabecularmeshwork.ThestainingofMMP_2wasstrongerthanTIMP_1.2.The expresslonofMMP-2andTI1,2increasedfrominfanttoadult(2～3 months),thendecreased.ConclusionMMP_2andTIMP_l,2wereprominently lden