9、TIMP-1在实验性自身免疫性肌炎中的表达及甲基强的松龙的影响.docVIP

9、TIMP-1在实验性自身免疫性肌炎中的表达及甲基强的松龙的影响 ?46? 文章编号:1003—2754(2006)01—0046—03 JApoplexyandNervousDiseases,February2006.Vol23.No.1 MMP一9,TIMP一1在实验性自身免疫性肌炎中的表达 及甲基强的松龙的影响 舒孔亮,张宁,肖波,申向民,李国良 摘要:目的探讨MMP一9在实验性自身免疫性肌炎(EAM)发病中的作用.以及甲基强的松龙对MMP一9, TIMP一1基因mRNA和蛋白表达的影响.方法应用RT—PCR及免疫组织化学技术.检测MMP一9,TIMP一1在 EAM组,甲基强的松龙治疗组(EAMM组)和对照组大鼠外周血,脾脏,肌肉组织中的表达水平.结果(1)EAMM 组大鼠发病程度低于EAM组,炎症细胞浸润和肌纤维坏死程度减轻.(2)MMP一9mRNA在EAM大鼠外周血,脾 脏淋巴细胞中的表达上调;MMP一9蛋白在EAM组大鼠肌肉组织表达增加.与对照组比较.差异有显着性.EAMM 组MMP一9mRNA及蛋白的表达均受到抑制,与EAM组比较差异有显着性.(3)与EAM组比较.EAMM组TIMP一 1基因mRNA表达明显增加,同时TIMP一1蛋白表达上调更明显.差异有显着性.结论MMP一9表达增加可能与 EAM发病有关;甲基强的松龙能减轻EAM发病程度.其作用机制可能与抑制MMP一9有关. 关键词:实验性自身免疫性肌炎;MMP一9;甲基强的松龙;RT—PCR;免疫组化;TIMP一1 中图分类号:R746.9文献标识码:A TheexpressionofMMP一9andTIMP一1inEAMandtheeffectsofmeIhylprednisoloneSHLKong—liang. ZHANGNing.XIAOBo.eta1.(ApartmentofNeurology,XiangyaHospital.CentralSouthUniz,ersity.Changsha 410008,China) Abstract:ObjectiveToinvestigatetheeffectofmatrixmetalloproteinase一9(MMP一9)intheattackofex— perimentalautoimmunemyositis(EAM)rats,andtheeffectsofmethylprednisoloneonexpressionsofmRNAand proteinofMMP一9andTIMP一1.MethodsTheexpressionlevelsofMMP一9andTIMP一1intheperipheralblood. spleenlymphocytesandmusclesweredetectedbyusingRT—PCRandimmunohistochemistrytechniquesandwas comparedamongEAMgroup,EAMwithmethylprednisolonetreatment(EAMM)groupandcontrolgroup.Re— suits(1)ThedegreeofepisodeinEAMMgroupwaslowerthanthatofEAMgroup,andtheinfiltrationofthein— flammatorycellsandthenecrosisofthemuscleswerealsomildinEAMMgroup.(2)Therewassignificantupregu— lationoftheexpressionoftheMMP一9genemRNAinthelymphocytesofperipheralbloodandspleenofEAMrats ascomparedwiththatofthecontro1.ElevationoftheexpressionofMMP一9proteininratsmusclestissuesof EAMgroupwasobservedobviously.However,theexpressionofthemRNAandtheproteinofMMP一9wassup— pressedsignificantlyinEAMMgroupcomparedwithEAMgroup.(3)Therewassignificantupregulationofthe expressionoftheTIMP一1genemRNAofEAMMratsascomparedwiththatoftheEAMgroup.Elevationofthe expressionofTIMP一1proteininratsmusclestissuesofEAMM