牛筋草离孺孢生物学特性和代谢产物活性测定.PDF

微生物学通报 MAY 20, 2009, 36(5): 666~671 Microbiology © 2009 by Institute of Microbiology, CAS tongbao@ 研究报告 牛筋草离孺孢生物学特性及代谢产物活性测定 杨 叶1* 王兰英1 胡美姣2 (1. 571737) (2. 571737) 摘 要: 对牛筋草离孺孢属(Bipolaris sorokiniana)NJ-08 菌株的生物学特性及代谢产物除草活性测 定结果表明：此菌株的菌丝体生长速度慢, 但产孢量大, 致病性强。菌丝体生长最适温度 25°C, 致 死温度是 55°C/10 min, 最适 pH 值为 8, 碳源以葡萄糖最佳, 供试氮源则不利于菌丝体生长。产生 分生孢子最适温度 25°C, 最适 pH 值为 8, 碳源以葡萄糖最好, 氮源以硝酸钠产孢量最高。分生孢 子萌发温度在 20°C~35°C 之间没有差异, 适宜 pH 值为 5~7, 碳源以 D-木糖的孢子萌发率最高, 氮 源以蛋白胨的孢子萌发率最高, 不同处理间存在明显差异。NJ-08 菌株产生的代谢产物作用于牛筋 草, 可使叶片黄化或枯死, 代谢产物对指示植物及杂草如柱花草、地桃花、猪屎豆和辣椒的胚根 胚芽生长都有明显的抑制作用, 抑制率均超过了 90%, 显示出很好的除草活性。 关键词: Bipolaris sorokiniana , 生物学特性, 除草活性 Biological Characteristics and Herbicidal Activity of Metabo- lites Produced by Bipolaris sorokiniana from Eleusine indica YANG Ye1* WANG Lan-Ying1 HU Mei-Jiao2 (1. Environment and Plant Protection College, Hainan University, Danzhou Hainan 571737, China) (2. Environment and Plant Protection Institute, CATAS, Danzhou, Hainan 571737, China) Abstract: The biological characteristics of Bipolaris sorokiniana strain NJ-08 and herbicidal activity its metabolites was tested. The results showed that NJ-08 displayed excellent pathoginicity and high sporulation. The optimum temperature and pH for mycelial growth was 25°C and 8 respectively, the lethal temperature was 55°C maintaining 10 min, the glucose was the best among carbon sources for mycelial growth but all nitrogen source did not significantly