depdc7基因在肝癌细胞中表达及其生物学作用word格式论文.docxVIP

优秀毕业论文 精品参考文献资料 8．Use the RT-PCR method to detect the DEPDC7 influence on the expression of related invasion gene. Results： 1．Through RT-PCR semi-quantitative analysis, the mRNA expression level of DEPDC7 gene in tumor cell significantly reduced，the differences between three groups of hepatocarcinoma cells are statistically significant (P0.05). 2 ． By Western Blot semi-quantitative analysis, the differences of protein expression levels between hepatic cell HL-7702 and three lines of hepatocarcinoma cells are statistically significant (P0.05). And the differences among three hepatocarcinoma cells are significantly different. the protein express level of Huh7 is the lowest, has statistical significance (P0.05). 3．Transfect the Hepatocarcinoma cells with the pEGFP-C1-DEPDC7 vector, it is found that the DEPDC7 protein is mainly located on the membrane and a little is found in cytoplasmic. 4．As the normal growth curve shows, hepatocarcinoma cells grow faster and be in logarithmic phase within 72hs, and the cells grow slower afer transfection of pIRES2-EGFP-DEPDC7 vector . 5．Overexpression of DEPDC7 gene can suppress the cell proliferation rate but can not cause apoptosis. 6．Overexpression of the DEPDC7 gene can suppress the invasion of tumor cells and influence the expression of gene matrix metalloproteinase. Conclusion： 1．The DEPDC7 gene express high in hepatic cell, low in hepatocarcinoma cells. The DEPDC7 protein is mainly located on the cell membrane,some appear in cytoplasm. 2．Upgrading the expression of the DEPDC7 gene can not cause cell apoptosis, but can suppress the rates of cell proliferation and invasion, the expression of MMP-9 decreased,and TIMP-1,TIMP-3 raised. Key words：gene chip, bioinformatics , hepatic cell, tumor cell, the DEP domain 3 3 Acr 英文缩略词表释义表 Acrylamide 丙烯酰胺 Bis Bisacrylamide N, N’-甲叉双丙烯酰胺 BSA Bovine serum albumin 牛血清白蛋白 DMSO Dimethylsulfoxide 二甲基亚砜 DEPC Diethypyrocarbonate 焦碳酸二乙酯 DNA deoxyribonucleic acid 脱氧核糖核酸 ddH2O dNTP double di