drp1在糖尿病大鼠坐骨神经中作用及白藜芦醇干预影响word格式论文.docxVIP

Meanwhile, series dosages of resveratrol were administered, then its effects on Drp-1 were assessed. The ultrastructure of the sciatic nerve from rats was observed by electron microscopy, and the effect of resveratrol on diabetic neuropathy was evaluated in the pathological point, from which we discussed the pharmacal effect of resveratrol on the diabetic neuropathy and its possible mechanisms.Methods: Seventy clean male SD rats were randomly divided into 2 groups: normal group was fifteen and streptozotocin treated group was fifty-five.There have forty-nine successful models in treated group and four models were removed randomly.The remaining forty-five models were randomly divided into diabetic group, low dose of resveratrol treated group (20mg·kg-1·d-1)and high dose of resveratrol treated group(100mg·kg-1·d-1), n =15. After 12 weeks, rats were sacrificed and sciatic nerves were taken. Part I. To observe the general condition of rats in each group, to measure blood glucose by roche blood glucose meter, to detect the expression of Drp-1 by Real-time PCR, and to detect the protein of Bax, Bcl-2and Caspase-3 by immunohistochemical staining. Part II. With the intervention of resveratrol to Drp-1, the effects were assessed, and the ultrastructure of sciatic nerve was observed by transmission electron microscope.Results:Weight and blood glucoseAt the end of the experiment, body weight of normal group increased generally, and blood glucose was normal. Compared with the normal group, body weight of the diabetic group and resveratrol treated group increased slowly, blood glucose increased significantly. The difference was statistically significant (all P 0.05). Compared with the diabetic group, weight and blood glucose of the resveratrol treated group were of no significant difference (P 0.05). Compared with the low dose of resveratrol treated group, weight and blood glucose of the high dose of resveratrol treated group were of no significant difference,either(P 0.05).Drp-