eppin优势表位自组装纳米避孕疫苗的构建及实验分析word格式论文.docx

hCGHumanchorionicgonadotropin人绒毛膜促性腺激素HPLCHighPerformanceLiquidChromatography高效液相色谱HRPHorseradishPerocidase辣根过氧化物酶ISCOMsImmunostimulatingcomplexes免疫刺激复合物IFN-γInterferon-γγ干扰素IgGImmunoglobulinG免疫球蛋白GILInterleukin白细胞介素kDaKilodalton千道尔顿KLHKeyholelimpethemocyanin锁蓝蛋白LLiter升LCLangerhanscells朗格汉斯细胞LHLuteinizinghormone黄体生成素LPSLipopolysaccharide脂多糖LSCMLaserscanningconfocalmicroscope激光共聚焦显微镜MAPMultipleantigenpeptide多聚抗原肽mBMDCMousebonemarrow dendritic cells小鼠骨髓树突状细胞MFIMedianfluorescenceintensity中位荧光密度minMinute分钟MHCMajorhistocompatibilityantigen主要组织相容性抗原mlMilliliter毫升molMole摩尔mMMillimole/liter毫摩尔/升MNMcroneedle微针MSMassSpectrometry质谱MWMolecularweight分子量ODOpticaldensity光密度PBSPhosphatebuffersolution磷酸缓冲生理盐水pgPicogram皮克PLAPolylacticacid聚乳酸PLGAPoly(lactic-co-glycolicacid)聚乳酸-羟基乙酸共聚物PSAProstatespecificantigen前列腺特异性抗原rhEppinRecombinanthumanEppin重组人EppinrpmRoundperminute转/分钟sSecond秒SAPNSelf-assemblingpolypeptidenanoparticle自组装多肽纳米颗粒SCSubcutaneous皮下Semg1Semenogelin精囊凝固蛋白TEMTransmissionelectronmicroscopy透射电子显微镜ThHelperTlymphocyte辅助性T淋巴细胞TLRToll-likereceptorToll样受体WAPWheyacidicprotein乳清酸蛋白ZPZonapellucida透明带SynthesizingofEppin peptideself-assembliesand study onitsimmunocontraceptiveeffectsAbstractInrecentyears,theresearchesindicatethatthedevelopmentofcontraceptivevaccinebasedonepididymalproteaseinhibitor(Eppin)hasmadesomeprogress,butstillhavethefollowingproblems:(1)the contraceptivevaccinebasedon thedominantfunctional epitopeofEppincanreducetheadversereactions,butpoorlyimmunogenic;(2)epitopecross-linkedcarrier protein can enhance immunogenicity,but the specificity of theantibodyreduced;(3)inanimalexperiment,thecontraceptivevaccinebasedonEppinalwaysneedimmuneadjuvants,suchasFreundsadjuvant,butitsnotsuitableforhuman.Therefore,thekeystepfor developingacontraceptive vaccineis to developa safer, more effectivedeliverysystem.Previousstudieshavealsoshownthatthedominantfunctionalepitopeof Eppincaninduceaspecificantibodyresponseandsuppressfertility.Forthisreason,aself-assembledpeptidenanofibersvac