egfr adam9在胰腺癌panc 1微球体细胞中表达的研究word格式论文.docx

Expression of EGFR andADAM9 in Pancreatic CancerPanc-1 Cell SpheresDepartmentofPancreatic-BiliarySurgery,TongjiHospital,TongjiMedicalCollege,HuazhongUniversityofScienceandTechnologypost-graduate:HongXiaoquanTutor:Pro.WangXinABSTRACTObjectiveThetheoryofcancerstemcells(CSCs)offerusanewthoughtabouttumors,moreandmorekindsofcancerstemcellswereisolatedandidentifiedformcorrespondingtissue.Our aimwasto isolate and identifyCSCs in Pancreatic cancercelllinesPanc-1 withserum-freemedium（SFM）includingepidermalgrowthfactor(EGF),basicfibroblastgrowthfactor(bFGF),insulinandB27supplement,andtodetecttheexpressionlevelofEGFRandADAM9byReal-timePCRandWesternblot.MethodsSelf-renewalassay,differentiationassay,detectionofsidepopulation(SP) cellswereperformedtocharacterizeCSCsinPanc-1mammospheresculturingwithserum-freemedium.Moreover,Real-timePCRandWesternblotwereusedtodetecttheexpressionofEGFRandADAM9.ResultsPanc-1passagedandformedmammospheresintheabsenceofserum.CellspheresdifferentiationoccurredwhenserumwassupplementedinSFM.SPcellsinPanc-1mammosphereswere(3.38±0.46)%,whichshowedincreasedexpressioncomparedwiththedifferentiatedcells(P0.05).TheexpressionofEGFRandADAM9mRNAwere2.5,3.0 timeshigherinmammospheresthaninthe differentiatedcells. respectively (P0.05).Western blot analysis suggested that EGFR and ADAM9 protein were expressed inmammospheresathigherlevelsthanthatinthedifferentiatedcells(P0.05).ConclusionsPancreaticcancercellspheresmaybeasourceforcollectingCSCsby culturingwithserum-freemedium.ADAM9mayplayanimportantroleintheoccurrenceanddevelopmentofPancreaticcancerthroughtheEGFRsignalingpathwayandADAM9isexpectedtobecomenewtherapeutictargetsforPancreaticcancer.Keywords Pancreaticcancerstemcells;mammospherecells;EGFR;ADAM9独创性声明本人郑重声明，本学位论文是本人在导师指导下进行的研究工作及取得的研究成果的总结。尽我所知，除文中已经标明引用的内容外，本论文不包含任何其他个人或集体已经发表或撰写过的研究成果。对本文的研究做出贡献的个人和集体，均已在文中以明确方式标明。本人完全意识到本人将承担本声明引起的一切法律后果。学位论文作者签名：日期：年月日学位论文版权使用授权书本学位论文作者完全了解学校有关保留、使用学位论文的规定，即：学校有权保留并向国家有关部门或机构送交论文的复印件和电子版，允许论文被查阅和借阅。本人授权华中科技大学可以将本学位论文的全部或部