erk信号通道在哮喘大鼠模型t淋巴细胞il13的产生中的调控作用分析word格式论文.docx

ObjectiveToinvestigatethechangesandactivationofextracellularsignal-regulatedkinase(ERK) signalingpathwayinasthmaticlymphocytes.Toexploretheroleofextracellularsignalregulatedkinase(ERK)intheexpressionofThcytokine,interleukin13(IL-13)bylymphocytesinasthma.MethodsandMaterials40SDratswererandomlydividedinto2groups，normalcontrolandasthmaticgroup. Peripheralbloodmonocytes(PBMCs)wereisolatedandpurifiedfrombloodofeachasthmaticSDratsanddividedinto5groups：control，asthmaticcells,asthmaticcellsstimulatedwithepidermalgrowthfactor(EGF)，orwithERKinhibitorPD98059,orwithPD98059 and EGF together. The expression of p-ERK protein was observed byimmunocytochemicalstaining，theexpressionofERKmRNAwasobservedbyRT-PCR,IL-13proteininsupernatantswasmeasuredbyELISA.Results1．Comparedtothepercentageofcellswithpositivep-ERKproteinofnormalcontrolgroup(3.09%±0.27%),thepercentageofcellswithpositivep-ERKproteinofasthmaticgroup(15.63%±1.04%）wassignificantlyincreased(n=5，p0.01).2．ComparedtotheexpressionofERKmRNAofnormalcontrolgroup(0.2396±0.034),the expression of ERK mRNA of asthmatic group (0.7119±0.052) was significantlyincreased(n=5，p0.01).3．Comparedtothepercentageofcellswithpositivep-ERKproteinofasthmaticgroup (15.63%±1.04%), the percentage of cells with positive p-ERK protein of EGF group（30.60%±1.96%)wassignificantlyincreased(n=5，p0.01),whilethepercentageofcellswithpositivep-ERKproteinofPD98059group(9.08%±0.38%)wasdecreasedsignificantly(n=5，p0.01).Thepercentageofcellswithpositivep-ERKproteinofasthmaticPD+EGFgroup(17.11%±1.51%)showednosignificantdifferencecomparedtothatoftheasthmaticgroup(n=5，p0.05).4．ComparedtotheexpressionofERKmRNAofasthmaticgroup(0.7119±0.052),theexpression of ERK mRNA of asthmatic EGF group (0.9606±0.075) was increasedsignificantly,whiletheexpressionofERKmRNAofasthmaticPD98059group(0.4547±0.037)wasdecreased significantly(n=5，p0.01).TheexpressionofERKmRNAofasthmaticPD+EGFgroup(0.7606±0.049)showednosignificantdifferencecomparedtothatofasthmaticgroup(n=5，p0.05).5．Comparedtotheexp