evc2在早期胚胎发育中的作用分析word格式论文.docxVIP

expression pattern of EVC2 in early chick embryos using in situ hybridization. And then we found a group of cells which co-expressed EVC2 and AP-2α in dorsal hindbrain by using fluorescence in situ hybridization and immunofluoresence. AP-2α is one kind ofcranial neural crest cell markers. To study the effect of EVC2 on cranial neural crest cells in detail we employed electroporation to overexpress or knockdown EVC2. At last, we expressed EVC2 protein to find the target molecular of EVC2 via CO-IP.In summary, we revealed that EVC2 plays an important role in early embryo development by affecting the migration of cranial neural crest cells. This finding will broaden our understanding of the mechanism how EVC2 mutants cause diseases, and help us to find the way for preventing and curing EvC syndrome and WAD. Conclusions : EVC2 plays an important role in early embryo development by affecting the migration of cranial neural crest cells.Key Word: EVC2; embryo; early development缩略语词表缩略语英文全称中文全称EvCEllis-van Creveld syndromeEvC 综合征WADWeyers acrodental dysostosisWeyers 颅面骨发育不全PBSPhosphate buffered saline磷酸盐缓冲液APES3-aminopropyltriethoxysilane氨基丙基三乙氧基硅烷BSABovine Serum Albumin牛血清白蛋白APAlkaline Phosphatase碱性磷酸酶HRPHorseradish Peroxidase辣根过氧化物酶EDTAEthylenediamine Tetracetic Acid乙二胺四乙酸二钠IFImmunofluorescence免疫荧光WBWestern blot免疫印迹法FISHFluorescence in situ hybridization原位杂交shRNASmall hairpin RNA小发卡 RNANCCNeural crest cell神经嵴细胞CNCCCranial neural crest cell颅面神经嵴细胞广东药学院学位论文原创性声明本人郑重声明： 本人所呈交的学位论文，系我个人在导师的指导下 进行研究工作所取得的成果。除文中已特别加以标注和致谢的地方外，不 包含其它个人或机构已经发表或撰写过的研究成果。对本研究做出贡献的 其它个人和集体，均已在文中明确说明和致谢。本人充分意识到本声明的 法律结果完全由本人承担。学位论文作者签名：＿＿＿＿＿ 日 期：年月日学位论文使用授权的声明本人完全了解广东药学院有关保留和使用学位论文的规定，学校有权 保留和向有关部门或机构送交本论文的复印件和电子版，允许论文被查阅 和借阅。学校可以将本学位论文的全部或部分内容编入有关数据库，可以 采用影印、缩印或其它复印手段保存和汇编本学位论文。保密论文在解密后适用本声明。论文作者签名：＿＿＿＿＿论文导师签名：＿＿＿＿＿日 期：年月前言Ellis- van Creveld 综合征(EvC)又名软骨-外胚层发育不良，是一种常染色体隐性遗 传病，其主要特征为短肢短肋，轴后多指（趾）[图 1][1]，生长迟缓，外胚层和心脏 图 1EvC 综合征中的骨骼发育异常(Ruiz-Perez VL et al,2009) 发育不良。研究表明 EVC 基因缺陷小鼠与 EvC 患者一样：短肢，短肋，牙齿发育