甲醛暴露所致工人早期遗传损伤的分析-analysis of workers early genetic damage caused by formaldehyde exposure.docxVIP

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甲醛暴露所致工人早期遗传损伤的分析-analysis of workers early genetic damage caused by formaldehyde exposure.docx

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甲醛暴露所致工人早期遗传损伤的分析-analysis of workers early genetic damage caused by formaldehyde exposure

华中科技大学硕士学位论文 IV IV biological marker of formaldehyde exposure and of effective dose. In this research, we used 215 workers employed in two plywood industries as our object of study, all subjects were interviewed with questionnaire and blood samples were collected. Air sampling and formaldehyde analysis were performed through HPLC according to the National Institute for Occupational Safety and Health (NIOSH) method in selected workshops, and serum FA-HSA were detected among all workers. The average concentration of FA in the low FA exposed workshop (0.58±0.20 mg/m3) is much lower than in the high FA exposed workshop (1.48±0.61 mg/m3), and the average concentration of serum FA-HSA is (69.22±15.37) pg/ml and (136.29±89.49) pg/ml respectively. As the rising of air formaldehyde concentrations, the concentrations of FA-HSA in serum also increase. Formaldehyde can cause cytogenetic damage in exposed workers. In this study, KCL-SDS assay and single cell gel electrophoresis were used to investigate DNA-protein crosslinks and DNA strand breaks in human peripheral blood lymphocytes, and cytokinesis-block micronucleus assay was used to detect chromosome damage. The results didn’t show any significant difference of cytogenetic damage among low-exposure, intermediate-exposure and high-exposure groups (P0.05), but it did show a positive correlation between working years and CBMN‰ (rs=0.339, P0.001) and OTM (rs=0.239, P=0.003). The workers whose working years were longer than 4.4 years had a higher CBMN‰ risk as well as a higher OTM risk compared to the workers whose working years were shorter than 1.5 years (Ptrend0.001). In summary, we selected 215 occupational FA exposed workers as our study object and investigate the association of FA exposure and cytogenetic damage in peripheral lymohocytes. The results suggested that FA-HSA in serum can be a biomarker of FA exposure and of effective dose. With the increase of working years, the DNA dam