itgb4bp调控tgf-β1表达的实验研究-experimental study on itg b4 bp regulating tgf - β 1 expression.docxVIP

PMSFPhenylmethyl Sulfonyl Fluoride苯甲基磺酰氟BCSBovine Calf Serum小牛血清HRPHorseradish peroxidase辣根过氧化物酶ODOptical density光密度PVDFPolyvinylidene fluoride聚偏二氟乙烯GAPDHGlyceraldehydes-3-phosphate dehydrogease甘油醛-3-磷酸脱氢酶SDSSodium Dodecyl Sulphate十二烷基磺酸钠PAGEPolyacrylamide gel electrophoresis聚丙烯酰胺凝胶电泳APAmmonium Persulfate过硫酸胺 TEMEDTetramethylethylenediamine四甲基乙二胺 SDstandard deviation标准差The transfactional regulation of ITGB4BP on profibrotic TGF-β1AbstractFibrosis is the end result of chronic tissue injury of organs induced by a variety of harmful stimuli, which is defined by the overgrowth, hardening, and/or scarring of tissues and attributed to excess deposition of extracellular matrix components. Progression of fibrosis involves interstitial hypercellularity, excessive matrix accumulation, and atrophy of epithelial structures leading to deformation and dysfunction ultimately.We previously compared differentially expressed genes between normal skin andhypertrophic scar with microarray and founded a targeted gene, P311, was highly expressed in the early hypertrophic scar[1-2]. P311 does not belong to any known family of proteins, It has been demonstrated that P311 could induce a TGF-β1–independent, nonfibrogenic myofibroblast phenotype, and that adenovirus-mediated P311 expression could accelerate nerve regeneration of the axotomized facial nerve[3-4]. To investigate the potential molecular mechanisms of P311 in the formation of hypertrophic scar, we screened the possible interactive protein with P311 from the adult liver cDNA library with a yeast two-hybrid system and acquired a qualified one--ITGB4BP [5].ITGB4BP (alias eIF6, p27BBP) was identified by its ability to bind β4 integrin[6]. ITGB4BP was present in the nucleolus of all kinds of cells. Data show that ITGB4BP was a necessary transacting factor for the biogenesis of eukaryotic 60S ribosomal subunits[7-9]. Meanwhile ITGB4BP was recruited by the RISC complex, enabling microRNA complexes to exert their repressive effe