分子印迹技术与lc-msms联用在食品中痕量四环素类检测的应用分析-application and analysis of molecular imprinting technique combined with lc - msms in the detection of trace tetracycline in food.docxVIP

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分子印迹技术与lc-msms联用在食品中痕量四环素类检测的应用分析-application and analysis of molecular imprinting technique combined with lc - msms in the detection of trace tetracycline in food.docx

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分子印迹技术与lc-msms联用在食品中痕量四环素类检测的应用分析-application and analysis of molecular imprinting technique combined with lc - msms in the detection of trace tetracycline in food

三．以四环素分子印迹聚合物作为固相萃取填料检测鱼肉、饲料、虾、猪肠衣、蜂蜜、猪肉等实际样品中痕量四环素类抗生素残留。系统研究了 LC/MS/MS 条件和固相萃取条件（包括活化、上样、淋洗和洗脱步骤），在最优固相萃取条件下，四环素类物质在 MIP 柱上的萃取回收率在 70.02%--89.66%之间，远远高于 NIP 柱，并且可以实现大体积进样。将固相萃取与 LC/MS/MS 联用，对加标的鱼肉、饲料、虾、猪肠衣、蜂蜜、猪肉等样品进行检测，加标回收率在 95.49%-104.8%之间，RSD 小于 10.3%，检测限可达 ng/g。关键词:分子印迹技术，固相萃取，液相色谱－串联质谱，四环素类抗生素AbstractTetracyclie antibiotics are intensively used in veterinary medicine and as food additives for growth promotion in the farming industry, and they can product negative effect for human body. So it is very important to develop a fast, high selective method for determination of tetracyclines in foodstuff samples.Molecular imprinting technique is now an established method that can be applied in separation procedures and chemical analyses, and the molecularly imprinted polymers(MIPs) have the characteristics of high ligand selectivity and affinity.Today, solid-phase extraction(SPE) is the most popular sample preparation method ,in order to enhance the selectivity, the use of MIPs as selective sorbents is becoming increasingly popular.Liquid chromatography tandem mass spectrometry (LC/MS/MS) technique frequently provide specific, selective and sensitive quantitative results, and allows unequivocal identification of trace contaminants in complex food matrices, which has become one of the major tools in food analysis.In this thesis, tetracyclies in different foodstuff samples were determined by LC/MS/MS coupled with molecularly imprinted solid-phase extraction (MISPE), and good results were obtained. The major topics in the thesis include:In this paper, the importance of food safety, the status of food analysis, the molecularimprinted technology, the basic principle of LC-MS/MS and its application in food analysis were systematically summed up. Synthetic methods of molecularly imprinted polymers and application of the LC/MS/MS in food analysis were particularly outlined.MIPs were prepared by precipitation polymerization with tetracycline as the templatemolecule. Effec