一氧化碳对肾脏缺血再灌注损伤的保护作用及其新机制分析-protective effect of carbon monoxide on renal ischemia-reperfusion injury and analysis of its new mechanism.docx

缩写及中英文对照英文缩写BSA英文全称Bovineserumalbumin中文全称牛血清白蛋白COHbCarboxyhemoglobin碳氧血红蛋白CoPPCo(III)ProtoporphyrinIXchloride钴原卟啉CORMCarbonmonoxide-releasingmolecule一氧化碳释放分子DABDiaminobenzidine3,3-二氨基联苯胺DAMPDamageassociatedmolecularpattern危险相关分子模式DEPCDiethylpyrocarbonate焦碳酸二乙酯DMSODimethylsulphoxide二甲基亚砜EDTAEthylenediaminetetraaceticacid乙二胺四乙酸ELISAEnzyme-linkedimmunosorbentassay酶联免疫吸附检测FACSFluorescenceactivatedcellsorter激发荧光细胞分离器FBSFetalbovineserum胎牛血清FITCFluoresceinisothiocyanate异硫氰酸荧光素GmeanGeometricmeanfluorescenceintensity几何均数平均荧光强度HATHistoneAcetyltransferase组蛋白乙酰基转移酶HBSSHank’sbalancedsaltsolution汉克氏平衡盐溶液HDACHistoneDeacetylase组蛋白去乙酰化酶HEHEPESHemotoxylinandeosin4-(2-hydroxyerhyl)piperazine-1-erhanesulfonicacid苏木精-伊红4-羟乙基哌嗪乙磺酸HMGB1HO-1High-mobilitygroupbox1HemeOxygenase1高迁移率族蛋白1血红素加氧酶-1HRPPeroxidase,Horseradish辣根过氧化物酶HSP-32Heatshockprotein-32热休克蛋白-32ILInterleukin白细胞介素IRIMCP-1MPOO2HbIschemiareperfusioninjuryMonocytechemoattractantproteinMyeloperoxidaseOxyhemoglobin缺血再灌注损伤单核细胞趋化蛋白-1髓过氧化物酶氧和血红蛋白ODOpticaldensity吸光值PAGEPolyacrylamidegelelectrophoresis聚丙烯酰胺凝胶PBSPhosphate-buffersaline磷酸盐缓冲液PCRPolymerasechainreaction聚合酶链锁反应PEPhycoerythrin藻红蛋白pHPowerofhydrogen酸碱度PMSFPhenylmethanesulfonylfluoride苯甲基磺酰氟RAGEReceptorforadvancedglycationend晚期糖基化终产物受体SDSSodiumdodecylsulfate十二烷基硫酸钠TEMEDN,N,N,N-TetramethylethylenediamineN,N,N,N-四甲基乙二胺TLRTNF-?TUNELToll-likereceptorTumorNecrosisFactor-?TdT-mediateddUTPnickendlabelingToll样受体肿瘤坏死因子?原位末端转移酶标记法主要实验仪器-80℃超低温冰箱ThermoBioimagingSystems凝胶图像分析系统UVPHERACELL24i培养箱ThermoScientificPCR仪（PTC0200）Bio-RadRealtime-PCR仪ABIsteponeplusWesternBlot电泳、转膜系统Bio-Rad冰冻切片机Leica超纯水净水装置（WATERPROPS)LABCONCO超净工作台苏州净化设备公司低氧培养箱HealForce电热恒温水槽（DK-8D）上海精密实验设备有限公司电子天平（PL203）METTLERTOLEDO二氧化碳细胞培养箱（MCO-18AIC）SANYO分子杂交箱（HB-1000）UVP高速低温离心机（5415R）Eppendorf光学倒置相差显微镜（CK40-12PH）Olympus光学电子显微镜（BX51TF）Olympus核酸电泳系统Bio-Rad流式细胞仪BDFACSAria石蜡切片机Leica手术显微镜Leica数显水浴摇床（MAXQ7000）ThermoScientific台式大容量高速离心机（5810R）Eppendorf台式酶标仪（GENios）TECAN微量移液器Eppendorf温控离心机Leica旋涡混匀器（MS1）IKA荧光倒置显微镜（TE2000U）Nikon制冰机（AF100）SCO