盐度胁迫对锯缘青的蟹体内腺苷三磷酸酶和磷酸酶活性的影响.doc

盐度胁迫对锯缘青蟹体内腺苷三磷酸酶和磷酸酶活性的影响 农业环境科学2007,26(3):1173—1177 JoumalofAgro—EnvironmentScience 盐度胁迫对锯缘青蟹体内腺苷三磷酸酶和 磷酸酶活性的影响 徐华,艾春香,林琼武,陈宇锋,沈圆虹 (厦门大学海洋学系,亚热带海洋研究所,福建厦门361005) 摘要:采用酶学分析的方法研究了盐度(分别为5,15,25,35)胁迫下锯缘青蟹鳃,肌肉和肝胰腺中腺苷三磷酸酶和磷酸酶活性变 化.结果表明,随着盐度降低,青蟹鳃中Na*,K~-ATPase,Caz+,Mg2+一ATPase活性均升高,随着胁迫时间延长,Na~,K+-ATPase活性呈现不 同的变化规律,且趋于平缓.青蟹肌肉中ACP活性随着盐度的升高而呈小幅度下降,而肝胰腺中ACP活性则呈小幅度升高,但各组 之间差异不显着(O.O5).不同胁迫时间同一盐度组青蟹肌肉中的ACP活性有升有降.青蟹肌肉,肝胰腺中AKP活性随着盐度升高 而升高:随着胁迫时间延长,同一盐度组青蟹肌肉中AKP活性均呈升高趋势,肝胰腺中AKP活性有升有降.由此可见,盐度对青蟹生 理生化影响显着. 关键词:锯缘青蟹;盐度;腺苷三磷酸酶;磷酸酶 中图分类号:X503.225文献标识码:A文章编号:1672—2043(2007)03—1173—05 EffectofSalinityStressontheActivitiesofATPaseandPhosphataseintheMudCrab(Scyllaserrata1 uHua,AIChun—xiang,LINQiong—wn,CHENYu-feng,SHENYuan—hong (DepartmentofOceanography,XiamenUniversity,InstituteofSubtropicalOceanography,Xiamen361005,China) Abstract:Themudcrab(Scyllaserrata)isakindofimportantmarlneculturespeciesinChina.Salinityisoneofthemostimportantenviron— mentalfactorswhichrelatescloselywiththedevelopmentofs.serrata.TheactivitiesofATPaseandphosphataseingil1.muscleandhep— atopancreasofSserrataweredeterminedunderdifferentsalinities(5,15,25,35)forunderstandingitsphysiological—biochemicalresponseto salinitystress.TheresultsshowedthattheeffectsofsalinitystressonNa.K-ATPaseactivityinthegillofS.serrataweresignificant(Plt;0.05).? BothNa+,K+-ATPaseandCaz+’Mg一ATPaseactivitiesincreasedwiththesalinitydecreasing.TheNa~,K一ATPaseactivitiesineachtreatment tendedtoreachaplatformwiththetimegoingon.TheACPactivitiesinmuscledecreasedslightlywiththesalinityincreasing.butincreased inhepatopancreasinsignificantlyf尸O.05).rheAKPactivitiesinbothmuscleandhepatopancreasincreasedwiththesalinityincreasing,and increasedwiththetimedurationinthesametreatment.BothACPandAKPactivitiesinhepatopancreaswerehigherthanthoseinmuscle. ThechangesofAKPactivitiesinhepatopancreasandmuscleseemedtoberelatedwithNa+,K+-ATPaseactivitieschangingingillunderdif_ ferentsalinities.Theseresultssuggestedthatthephysiological-biochemicalresponseofS.serratatosa