不同浓度尼美舒利对皮肤伤口愈合的干预分析-analysis of different concentrations of nimesulide on skin wound healing.docxVIP

4. Observe and evaluate the wound healing process by observing angiogenesis, scar tissue of the skin, the skin stretched, immunohistochemical methods, inflammatory cells, the growth of fibroblasts et cetera. And using digital software analyze the healing process and scars. Using statistical methods analyze the results.ResultsCell culture experiments showed that COX-2 inhibitor had a significant effect on inhibiting the migration of fibroblasts, and the inhibit level were positively correlated to the concentration of COX-2 inhibitor, the experimental group using 25μ mol / L nimesulide has better results of cell growing comparing to other groups: cells can evenly migrate into the scratches gap and did not appear order chaos.Vivo experiments show: Through the intervention of COX-2 signaling pathways usingnimesulide, the skin wound healing process has been significant impacted. Using appropriate concentration of nimesulide can inhibit wound inflammation and control the migration of fibroblasts in the right level. Comparing the scar area of experimental group and the control group, we found statistical significance between the experimental groups and the control group on the incidence of hypertrophic scars, pigmentation, scar size and other aspects. The 25μ mol / L nimesulide group received the best healing results.Using Immunohistochemistry method to analyze the healing wound showed: In vitro applications, Nimesulide could significantly inhibit the expression of COX-2 in inflammatory cell, and the inhibition is positive correlation to the drug concentration.Statistical analysis indicated that there was statistically difference between the experimental group and control group, and statistical differences were significant between most of the experimental groups.ConclusionUsing Nimesulide in vitro applications on mouse surgical wound can intervene the expression level of COX-2 regulation of the inflammatory cells, COX-2 expression level has a negative correlation with the