水稻小分子热激蛋白基因oshsp17.8和锌指蛋白基因oszrl的表达分析及功能鉴定-expression analysis and functional identification of rice small molecule heat shock protein gene os hsp 17.8 and zinc finger protein gene oszrl.docxVIP

PAGE PAGE IV III III OsZRL 参与赤霉素、脱落酸信号途径。因此 推测锌指蛋白 OsZRL 是受赤霉素、 脱落酸调节的水稻生长发育负调控因子。 综上所述，水稻小分子热激蛋白 OsHSP17.8 基因在水稻热、旱和盐胁迫应答 中起了重要的作用，过表达 OsHSP17.8 基因可提高水稻的抗热、抗旱和抗盐能力； 水稻锌指蛋白 OsZRL 基因在水稻的根系发育调控中具有重要的功能，是水稻生长 发育的负调控因子。通过转基因技术分别将 OsHSP17.8 和 OsZRL 基因转入水稻， 用作基因改良，为培育水稻新品种、改善田间性状奠定基础。 关键词：小分子热激蛋白，锌指蛋白转录因子，非生物胁迫，抗逆性，根系发育 ABSTRACT Rice is one of the most important crops. With global warming and the greenhouse effect, abiotic stresses such as heat, drought has become an important factor affecting the rice production. Heat stress does harm to rice, causing rice to take heat shock response which induce of the expression of related genes, keep the stabilization intracellular environment to defense the harm of heat stress. The small heat shock protein is a class of molecular chaperone family plays an important role in intracellular protein folding, degradation and operation of the process. sHsps can enhance the resistance of plants. In the present work, OsHSP17.8, a small heat shock protein gene, was cloned and used to construct overexpression vector pHB-OsHSP17.8. The vector was introduced into Oryza sative L. Nipponbare by Agrobacterium-mediated transformation. Some positive transgenic plants were obtained and the function of OsHSP17.8 was analyzed. Real-time RT-PCR revealed a significant increase of OsHSP17.8 expression levels in overexpression transgenic lines compared with wild-type plants. The gene is constitutively expressed in mature rice, and has a highest expression abundance in flower than any tissue else. The expression pattern showed that OsHSP17.8 was highly expressed in treated with heat, drought, cold, salt and GA, NAA, ABA, especially sensitive to heat stress. Phenotype of mature transgenic plant showed that sterile grain rate is low, seed grain length and width is greater than the wild-type. Furthermore，the Heading Date in Rice was just at a high temperature, so we speculate OsHSP17.8 gene can enhance heat resistance. Compared with wild type, transgenic