石蒜悬浮细胞系的建立及其生物碱合成途径调控的分析-establishment of lycoris radiata suspension cell line and analysis of its alkaloid synthesis pathway control.docxVIP

ofalkaloidreduceinthebeginning.Thealkaloidcontentincreasedinthelatestageofthegrowthphase.Intheendofthecycle,thealkaloidcontentwasapproximatelyequaltothebeginningofcalluscycle.EstablishmentofeffectiveregenerationsystemofLycorisradiate.TheoptimalmediumsforbuddingofcalluswasMS+6-BA2mg·L-1+2,4-D1mg·L-1,andhadmorequantityofsmallbulbs.TheoptimalmediumsforrootingofsmallbulbswasMS+NAA3mg·L-1,andtheratecanreach100%.Theplantspropagationcoefficientcancreasedependoncallus,andthecyclecanreduce.Theplantsgrewthwellafteracclimatizingandtransplanting.TheestablishmentofcellsuspensionofLycorisradiate.Somefactorsforcellsuspensionhadbeenstudiedsuchasinoculumsamount,hormone,inositolandconditionculture.Wediscoveredthebestmethodofcellsuspensionwhichinoculunsamountwas15percentage,themediumwasMS+2,4-D0.5mg·L-1+KT0.1mg·L-1,inositolamountwas300mg·L-1.Thecellsuspensionhadgreatquality,thecellsuspensionhadgreatdispersion,thecellviabilitywashigh,celldivisionwasfast.Themediumfromgrowthphasecanreducethecellsuspensioncycle,andthecyclewas7days.ThecellsuspensioncurveswasS-shaped,andthecyclecandivideintolagphase,logarithmicgrowthphaseandstablephase.Thealkaloidcontentofcellsuspensionwashigh.TheGalanthaminecontentwas4.18timesofthebulbofLycorisradiateinfield.Effectsofalkaloidcontentofcellsuspensionforaddingprecursors.Someresearchhaveconfirmed,whichalkaloidofLycorisradiatehasthesameprecursors,phenylalanineandtyrosine.Addingphenylalaninehadonsignificantofalkaloidcontent.Addingtyrosinehadsignificantcomparedthecontrolgroup,tyraminetoo.Addingphenylalanineandtyrosinetogethercouldincreasealkaloidcontent,buthadnosignificantcomparedtoaddtyrosineonly.Keywords:Lycorisradiate,tissueculture,callus,cellsuspension,precursor,alkaloid独创性声明本人声明所导.交的论文是我个人在导州i指导F进行的研究工作必取得的研究成果。尽我所知，除了文中特别加以标注和致谢的地方外，论文中不包含其他人己经发表或撰写过的研究成果，也不包含为获得安徽农业入学或其它教育机构的学位成ìiEH而使用过的材料。与我才司工作的同志对本研究所做的任何贡献均已在论文巾作了明确的说明并表及了谢意.}研究尘签名A鱼龙蜘时间:如r(午4月(171-1关于论文使用授权的说明I本人完全了解安徽农业太学有关保留、使用学位论文的规定，即:学校有仪保留送交论文的u印件和磁盆，允许论文被查阅和借阅，口以采用影印、缩印或扫描等复制手段保存、汇编学位论文。同志安徽农业大