甜菜抗丛根病细胞生物学特征分析—着重于活性氧代谢及细胞壁成分的分析-analysis of biological characteristics of sugarbeet cells against clump root disease - focusing on analysis of active oxygen metabolism and cell wall components.docx

included the epidermal destruction, the fracture of the cortical parenchyma tissue, the shed in most cell and the damage of the wood parenchyma cells near the xylem, the protection for the roots loss completely. The performance of the susceptible beet is more serious than the resistant beet. In the susceptible beet, the cortical parenchyma cells were almost all off, growing slowly and the root’s development was blocked. However, there are no abnormal changes in the morphological anatomical structure of the beet vein. In addition, the resistant varieties have a well developed vascular bundle structure than susceptible varieties. When observed from the sub-cellular level, we found that BNYVV resulted in a series of damaged on cell ultrastructure of the susceptible beet, such as the deformation, empty and the structure of the nuclear is disorder, the mitochondria and the Golgi apparatus was significantly increased, small and medium-sized vacuoles were increased in the cytoplasmic. We observed some round or oval vesicles containing thin filaments broke into the vacuole at the edge of the vacuolar membrane. In the Vein cells the chloroplast disintegrated completely and a abundance of Osmiophilic globule emerged. Destruction of the cell ultrastructure of resistant varieties was lighter, a series of significant structural defense reaction produced in: the host cells: there was sediments in the cell wall and black granular sediment showed in the vacuolar membrane. These indicated that the description of the morphological changes of the tissue and the pathological changes of the organelle were relative to the resistance to rhizomania. We have utilized Physiological and biochemical methods and electron microscopy cytochemistry labeling technique to study the time-varying characteristics and the orientation of spatial distribution of the H2O2 and O2-· generated between the process of the beet- BNYVV interaction. A large number of H2O2 and O2-· detected both in the resistant