稀土掺杂类普鲁士蓝化学修饰电极在ce-ecl分析技术中的应用分析-application analysis of rare earth doped prussian blue chemically modified electrode in ce - ecl analysis technology.docxVIP

Abstract Abstract 研究生姓名：任小娜导师：马永钧 研究生姓名：任小娜 导师：马永钧 教授 专业：分析化学 研究方向：环境分析化学 IV capillary electrophoresis. The fundamental theory of CE and its isolation mode and detection technology are introduced simply. In the second section, the electrochemiluminescence (ECL) detection is summarized in detail, pay attention to the mode of the electrochemiluminescence of Ru(bpy)32+. The present research of CE-ECL and its application in analysis of traditional Chinese medicines and western medicines are introduced in the third section. The fourth section summarize the chemically modified electrode and pay more attention to the application of chemically 2+ modified electrode in improving the luminescence efficiency of Ru(bpy)3 . At last, the purpose and meaning of this work are discussed. Totally 124 references are cited in the chapter one. Chapter Two The study of evaluation on improving analytical sensitivity of CE-ECL using Eu-PB modified platinum electrode Our work explored the feasibility of improving the sensitivity of CE-ECL detection method by applying chemically modified electrode. An Eu-PB modified platinum electrode was prepared as working electrode, good catalytic oxidation 2+ characteristic for Ru(bpy)3 in the relatively lower potential(1.20 V vs. Ag/AgCl) was revealed and it has shown good reproducibility and stability. Tripropylamine (TPA) and proline (Pro) were made as labelled molecular for the evaluation of CE-ECL system using Eu-PB modified platinum electrode. Compared with bare platinum electrode, the absolute ECL intensity of TPA and Pro increased 3 times and linear range widened one order of magnitude, 5×10-10~1×10-5 M for TPA and 1×10-7~1×10-5M for Pro respectively, when Eu-PB modified platinum electrode was used in CE-ECL system. Based on three times the signal to noise ratio, the detection limits reduced to 1.29×10-10 M for TPA and 5.30×10-8 M for Pro. All experimental datas indicated that the sensitivity of CE-ECL detection s