BALBc小鼠阴虚型过敏性哮喘模型建立与评价.docVIP

BALBc小鼠阴虚型过敏性哮喘模型建立与评价 　　摘要：目的 建立BALB/c小鼠阴虚型过敏性哮喘模型并进行评价。方法 采用腹腔注射与皮下注射卵清白蛋白（OVA）致敏、雾化吸入OVA激发的方法复制过敏性哮喘BALB/c小鼠模型，激发后期合并灌胃甲状腺素复制阴虚型过敏性哮喘模型。在监测饮食、饮水及体质量的基础上，对支气管激发过程中哮喘行为学、肺功能及肺组织病理学等哮喘相关指标进行观测，同时对cAMP、cGMP及肺液清除功能等阴虚证相关指标进行考察。结果 阴虚哮喘模型组小鼠出现明显的哮喘症状，哮喘行为学综合评分明显升高，吸气峰流量、呼气峰流量、潮气量明显下降，呼吸频率增加，肺组织病理学可见明显的炎症反应；同时，阴虚哮喘模型组小鼠进食与饮水量明显增加，而体质量增长缓慢，肺组织湿重/干重比值下降，血清cAMP及cAMP/cGMP升高，cGMP下降，显示小鼠处于阴虚而阳相对亢盛的状态。结论 在OVA致敏、激发的基础上给予甲状腺素，可成功复制BALB/c小鼠阴虚型过敏性哮喘模型。 　　关键词：阴虚型过敏性哮喘；动物模型；BALB/c小鼠 　　DOI：10.3969/j.issn.1005-5304.2016.09.015 　　中图分类号：R228；R259.622.5；R332 文献标识码：A 文章编号：1005-5304（2016）09-0061-04 　　Establishment and Evaluation of BALB/c Mouse Model of Anaphylactic Asthma with Yin-deficiency Syndrome WANG Zhi-wang1，2， TUO Hai-yan1， REN Yuan1，2， LI Rong-ke1， LIU Xue-feng1， CHENG Xiao-li1 （1. Gansu University of Chinese Medicine， Lanzhou 730000， China； 2. Key Laboratory of Pharmacology and Toxicology for TCM of Gansu Province， Lanzhou 730000， China） 　　Abstract： Objective To establish and evaluate a BALB/c mouse model of anaphylactic asthma with yin-deficiency syndrome. Methods Ovalbumin （OVA） was injected to sensitize and was inhaled to stimulate to replicate the BALB/c mouse model of anaphylactic asthma. Thyroxin was used for gavage during late stimulation to replicate the BALB/c mouse model of asthma with yin-deficiency syndrome. On the basis of monitoring food and water intake and body weight， asthma related indexes， such as asthmatic behaviors， respiratory function， lung histopathology， were detected， and yin-deficiency syndrome related indexes， such as cAMP and cGMP， and pulmonary fluid clearance were examined. Results The BALB/c mouse model of asthma with yin-deficiency syndrome showed obvious asthma symptoms； comprehensive scores of asthmatic behaviors increased significantly； inhaling peak flow， exsufflation peak flow， and tidal volume decreased significantly； lung tissue histopathology showed obvious inflammatory response. Meanwhile， food and water intake of BALB/c mouse mode