MMP―2P53蛋白在大鼠实验性蛛网膜下腔出血后早期脑损伤中作用研究.docVIP

MMP―2P53蛋白在大鼠实验性蛛网膜下腔出血后早期脑损伤中作用研究 　　【摘要】 目的：建立大鼠蛛网膜下腔出血（SAH）动物模型，检测脑组织中MMP-2及P53蛋白的表达水平及脑组织细胞凋亡情况，探讨MMP-2和P53蛋白在蛛网膜下腔出血后早期脑损伤中的作用机制。方法：72只SD大鼠随机分成假手术组、实验组。实验组又分为出血后12 h、24 h、48 h、3 d、5 d共5个时相组，每个时相组各12只大鼠。各组标本分别检测MMP-2及P53蛋白在海马CA1区的表达水平，海马CA1区神经细胞凋亡情况。结果：实验组海马CA1区MMP-2、P53蛋白表达及凋亡细胞数较假手术组均有增高，以24、48 h组更为显著。结论：MMP-2和P53蛋白在大鼠蛛网膜下腔出血后脑组织中表达明显增多，神经元细胞凋亡显著，两者与早期脑损伤关系密切，可能与MMP-2降解细胞外间质，破坏血脑屏障，导致血脑屏障通透性增高，P53蛋白诱导神经细胞凋亡有关。 　　【关键词】 蛛网膜下腔出血； MMP-2； P53蛋白； 细胞凋亡 　　【Abstract】 Objective：To study the MMP-2 and P53 in the role of early brain injury after subarachnoid hemorrhage preliminary by detecting MMP-2 and P53 protein expression level and apoptosis in brain tissue in order to provide a theoretical basis for treatment of subarachnoid hemorrhage in clinical practice.Method：72 clean-level male SD rats were randomly divided into sham-operated group（n=12） and SAH group（n=60）.SAH group were randomly divided into 12 h，24 h，48 h，3 d and 5 d group，12 rats in each group.The MMP-2 and P53 protein expression levels and neurocyte apoptosis in hippocampus CA1 were detected.Result：The MMP-2，P53 protein and the number of apoptotic cells in hippocampus CA1 of SAH group in each time were increased compared with those of sham-operated group，the differences were the most significant at the 24 and 48 h group.Conclusion：MMP-2 and P53 protein are closely related to early brain injury after subarachnoid hemorrhage by the former，MMP-2 increasing the permeability of blood-brain barrier by degradation of extracellular matrix to destroy blood-brain barrier，and the latter P53 protein，inducing the neurocyte apoptosis. 　　【Key words】 Subarachnoid hemorrhage； MMP-2； P53 protein； Apoptosis 　　First-author’s address：Liaocheng Central Hospital of Shandong Province，Liaocheng 252000，China 　　doi：10.3969/j.issn.1674-4985.2017.25.008 　　蛛?W膜下腔出血（subarachnoid hemorrhage，SAH）是神经系统常见的急危重症，年发病率为（2～16）/10万[1]，且近30年来发病率呈上升趋势[2]，最常见的病因是颅内动脉瘤破裂，约占所有自发性蛛网膜下腔出血发病原因的34%[3-4]，约35%患者在SAH后24 h内死亡，与颅内动脉瘤破裂出血导致的早期脑损伤密切相关[5-6]