p16和Ki―67蛋白,TLR3在宫颈病变分流诊断中应用.docVIP

p16和Ki―67蛋白,TLR3在宫颈病变分流诊断中应用 　　[摘要] 目的 评估p16和Ki-67蛋白，TLR3检查在宫颈病变分流诊断中的临床价值。方法 整群选取该院2014年10月一2016年8月妇产科门诊阴道镜下活检病理诊断各类宫颈病变153例分别进行p16和Ki-67，TLR3检测。采用免疫组化技术（S-P法）检测p16及Ki-67 ，TLR3的表达。结果 在慢性宫颈炎、CINI级、CINⅡ级、CINⅢ级中，p16阳性率为13.21%、58.24%，96.25%、97.51%，Ki-67阳性率为10.05%、61.22%、94.12%、96.05%，TLR3阳性率为11.04%、62.32%、95.02%、97.05%，p16与Ki67，TLR3阳性率呈递增趋势，差异有统计学意义。结论 P16和Ki-67， TLR3对宫颈病变分流诊断有重要辅助诊断价值，精确指导宫颈病变的诊断与治疗，预防宫颈癌的发生。 　　[关键词] P16；Ki-67；TRL3；宫颈病变；筛查 　　[中图分类号] R711 [文献标识码] A [文章编号] 1674-0742（2016）10（a）-0026-03 　　Application of p16，Ki-67 Protein and TLR3 in Shunt Diagnosis of Cervical Lesions 　　LI Yan-xia 　　Gynecology of the Second People’s Hospital of Liaocheng， Affilicated to Taishan Medical Sciences Univercity，Liaocheng，Shandong Province，252601 China 　　[Abstract] Objective To evaluate the application of p16， Ki-67 protein and TLR3 in shunt diagnosis of cervical lesions. Methods Group selection from October 2014 to August 2016 153 specimens onfirmed by pathological diagnosis of cervical lesions and can not determine the level of cervical lesions after Biopsying under colposcope. p16，Ki-67，TLR3 were detected with Envision methode. Results In chronic cervicitis， CINⅠ， CINⅡ， CINⅢ， the positive expression rate of p16 for 58.24% and 13.21% and 96.25% and 97.51%， the positive rate of Ki-67 was 10.05% and 61.22% and 94.12% and 96.05%， TLR3 positive rate was 11.04%， 62.32%， 95.02%， 97.05%， p16 and Ki-67，TLR3 positive rate was increased， the difference is significant. Conclusion P16 and Ki-67， TLR3 is important auxiliary diagnostic value for shunt in the diagnosis of cervical lesions， accurate guide the diagnosis and treatment of cervical lesions， prevent the happening of the cervical cancer. 　　[Key words] P16；Ki-67；TRL3；Cervical lesions；Screening 　　宫颈癌是妇科肿瘤中仅次于乳腺癌的第二大恶性肿瘤[1]。目前，中国常用TCT结合HPV病毒检测-阴道镜检查-宫颈组织病理活检，对宫颈癌前病变有效地进行宫颈LEEP刀手术，宫颈锥形切除术，仍存在过度治疗问题[2]。随着生物标志物的日趋被关注，细胞周期抑制因子p16 蛋白，核增殖抗原Ki-67，Toll 样受体3（TLR3）对宫颈高低级别病变鉴别有一定的指导意义。该文分析研究2014 年10月―2016 年8月该院收治的153例子宫颈炎、宫颈癌前病变中p16蛋白，