TNF―α在强直性脊柱炎患者血清中表达水平及临床意义探讨.docVIP

TNF―α在强直性脊柱炎患者血清中表达水平及临床意义探讨 　　摘要：目的 通过检测强直性脊柱炎（AS）患者血清中TNF-α的表达水平，探讨其在AS发病中的临床意义。方法 分别测定AS活动期患者、AS非活动期患者及健康人血清TNF-α、CRP、ESR水平。结果 AS活动期患者、AS非活动期患者血清TNF-α水平均显著高于健康人，其差异有统计学意义（P0.01）；AS活动期患者血清TNF-α水平显著高于AS非活动期患者，其差异有统计学意义（P0.05），且TNF-α的表达与CRP呈正相关，与ESR无明显相关性。结论 TNF-α是与AS发病相关的细胞因子，可作为AS的实验室诊断指标以及AS活动期临床疗效的监测指标。 　　关键词：强直性脊柱炎；TNF-α ；酶联免疫吸附试验 　　The Expression of TNF-α in Patients Serum with Ankylosing Spondylitis and Clinical Significance 　　PENG Qi-yan 　　（Department of Clinical Laboratory，Wuhan University of Science and Technology Hospital， Wuhan 430070，Hubei，China） 　　Abstract：Objective To discuss the clinical significance of TNF-α by detecting the expression of TNF-α in patients with ankylosing spondylitis. Methods The levels of serum TNF-α， CRP， ESR were determined respectively in the AS active patients， the AS inactive patients and the healthy people. Results The contents of TNF-α in the AS active patients and the AS inactive patients were obviously higher compared with healthy people with statistical significanc（P0.01）；TNF-α in the AS active patients were obviously higher than the AS inactive patients with statistical significanc（P0.05）；the expression of TNF-αand CRP were positively correlated， but no obvious correlated with ESR. Conclusion The TNF-αis a cytokine associated with the occurrence of the AS， and it can be used as the laboratory diagnosis indicators， as well as the monitoring indicator of clinical curative effect in AS activity. 　　Key words：Ankylosing spondylitis；TNF-α；Enzyme-linked immunosorbent assay 　　??直性脊柱炎（ankylosing spondylitis， AS）是一种慢性进行性炎性疾病，主要累及中轴关节[1]，其主要症状表现为反复发作的腰痛、腰骶部僵硬、脊柱强直或畸形，该病起病隐匿且病程较长，容易误诊[2]。其具体发病机制目前仍未明确，可能与遗传、环境及免疫因素等有关，且临床上尚缺乏特异性治疗，仅依靠日常锻炼和一些药物缓解急性发作[3]。因此，探讨AS发病机制、早期诊断和及时治疗成为研究热点。肿瘤坏死因子α（tumor necrosis factor-α， TNF-α）是体内炎症反应和一系列病理生理过程的重要递质，有学者认为，TNF-α是AS中介导炎症反应的重要细胞因子[4]，因此，本研究通过检测AS患者血清TNF-α水平，探讨其在AS病程中的临床意义，旨在为AS的发病机制和治疗提供实验依据。 　　1资料与方法 　　1.1一般资料 选取2015年3月～2016年6月在我院就诊的门诊及住院AS患者共62例，其中AS活动期患者32例，包括男22例，女10例，年龄20～49岁；AS