Th17细胞相关细胞因子和抗CCP抗体检测在类风湿性关节炎中临床应用性研究.docVIP

Th17细胞相关细胞因子和抗CCP抗体检测在类风湿性关节炎中临床应用性研究 　　[摘要]目的 探讨Th17相关细胞因子和抗CCP抗体在类风湿性关节炎（RA）中的相关性和临床意义。方法 回顾分析2015年1月～2016年5月在我院治疗的60例RA患者，根据DAS28积分分为活动组（DAS283.2）32例和稳定组（DAS28≤3.2）28例，另选择60例健康者作为对照组，用ELISA法测定血清Th17相关细胞因子（IL-17、IL-6、TGF-β1），用胶体金免疫层析法检测抗CCP抗体阳性率，比较三组测定结果。结果 抗CCP抗体阳性率：活动组稳定组对照组（P稳定组对照组（P0.05）。TGF-β1水平：活动组稳定组对照组（P0.05）。结论 抗CCP抗体阳性率、IL-17、IL-6水平越高，RA的DAS28积分越高，而TGF-β1水平越高，RA的DAS28积分越低，因此，Th17相关细胞因子和抗CCP抗体可以提高RA诊断及活动性判断的灵敏度和特异度，为治疗新策略提供依据。 　　[关键词]Th17相关细胞因子；抗CCP抗体；类风湿性关节炎；临床意义 　　[中图分类号] R593.22 [文献标识码] A [文章编号] 1674-4721（2017）04（c）-0153-03 　　[Abstract]Objective To investigate the correlation and clinical significance of Th17-related cytokines and anti-CCP antibodies in rheumatoid arthritis.Methods 60 patients with rheumatoid arthritis（RA） who were treated in our hospital from January 2015 to May 2016 were retrospectively analyzed.According to DAS28 scores，the patients were divided in to active group of 32 patients（DAS283.2） and stable group of 28 patients （DAS28≤3.2）.Another 60 healthy subjects were selected as the control group.Serum Th17-related cytokines （IL-17，IL-6，TGF-β1） were determined by ELISA，and the positive rate of anti-CCP antibody was detected by colloidal gold immunochromatography.The testing results were compared between the three groups.Results The positive rate of anti-CCP：active groupstable groupcontrol group（Pstable groupcontrol group（P0.05）.TGF-β1 level：active groupstable groupcontrol group （P0.05）.Conclusion The higher of the positive rate of anti-CCP antibody，the level of IL-17 and IL-6，the higher DAS28 score of RA，and the higher of level of TGF-β1，the lower of the DAS28 score of RA.Therefore，Th17-related cytokines and anti-CCP antibodies can improve the sensitivity and specificity of RA diagnosis and activity judgment，providing the basis for new treatment strategies. 　　[Key words]Th17-related cytokines；Anti-CCP antibody；Rheumatoid arthritis；Clinical significance 　　类风湿性关节炎（rheumatoid arthritis，RA）是一种以炎性滑膜炎为主的系统性疾病，目前发病机制尚未阐明，近年发病率不断攀升[1]。其