中药上消合剂对大鼠急性放射性食管损伤上皮细胞凋亡影响实验研究.docVIP

中药上消合剂对大鼠急性放射性食管损伤上皮细胞凋亡影响的实验研究 　　摘 要:目的:观察中药上消合剂对大鼠急性放射性食管损伤中上皮细胞凋亡的影响并探讨其作用机制。方法:将100只雄性SD大鼠随机均分为空白组、造模组、生理盐水组、氨磷汀组及中药上消合剂组。用6MV-X射线对大鼠上段食管单次照射43Gy,分别于照射后第7天及第14天各组随机抽取10只大鼠取材,检测食管上皮细胞调亡指数、 Bcl-2和bax基因的表达及外周血TNF-α的水平。结果:造模后第7天及第14天大鼠食管上皮细胞中凋亡明显增加。与造模组相比,中药上消合剂组食管上皮细胞凋亡明显减少(P0.01),Bcl-2的表达及Bcl-2/Bax值显著增高(P0.05);外周血TNF-α的水平明显下降(P0.05)。结论:中药上消合剂可减轻急性放射性食管损伤中食管上皮细胞的凋亡。作用机制可能与抑制TNF-α的表达、上调Bcl-2的表达有关。 　　关键词:辐射性食管损伤;上消合剂;凋亡;大鼠 　　中图分类号:R285.5 文献标识码:A 文章编号:1673-7717(2010)01-0196-04 　　 　　Experimental Study on the Impact of Chinese Medicine Shang Xiao Decoction to the Epithelial Cell Apoptosis of Radiation Esophageal Injury of Rats 　　ZHOU Xia,ZHANG Gu,LAI Xiao?jin,WANG Yue?zheng,ZHENG Xiao,MA Sheng?lin 　　(Zhejiang Cancer Hospital,Hangzhou 310022,Zhejiang,China) 　　Abstract:Objective:To observe the effect of shang Xiao decoction(SXD) to the epithelial cell apoptosis of radiation esophageal injury of rats and to investigate the mechanism. Methods:One hundred SD male rats were randomly divided into 5 groups: blank control group,model group, saline group, Amifostine group and SXD group. The upper paragraph esophaguses of all rats except those in the blank group were given single dosese of radiation of 43Gy by 6MV-X ray. 10 rats in each group were randomly killed humanely on the 7th and 14th day after irradiation.Then the esophaguses were removed and sectioned.The esophageal epithelial cell apoptosis, Bcl?2 and bax gene expression and TNF-α levels of Peripheral blood were detected.Results:on the 7th and 14th day after irradiation,the cell apoptosis in esophageal epithelial tissue was markedly increased. Compared with the model group,the epithelial apoptotic cell was reduced significantly in esophageal mucosa of the SXD group (P0.01). The Bcl?2 protein expression and Bcl?2/Bax ratio were also higher Significantly(P0.05) and the TNF-α levels of Peripheral blood was lower Obviously in the SXD group than in the model group(P0.05).Conclusion:Traditional chinese medicine shang