乳香没药挥发油对川芎中阿魏酸促透机制研究お.docVIP

乳香没药挥发油对川芎中阿魏酸促透机制的研究お 　　[摘要] 该研究拟探索乳香没药挥发油对川芎的促透机制，观察乳香没药挥发油对角质层微观及分子结构的影响。通过测定川芎中阿魏酸在乳香没药挥发油作用下的角质层/介质分配系数，采用扫描电镜观察乳香、没药挥发油对皮肤角质层结构的影响，利用傅里叶变换红外光谱仪考察乳香、没药挥发油对角质层内角蛋白和脂质的分子结构的影响，来探索乳香没药挥发油对角质层微观及分子结构的影响。研究结果显示乳香、没药挥发油可使阿魏酸在角质层与介质分配增强，使角?|层有规律的，整齐紧密堆积排列的叠瓦式结构受到一定程度的破损，同时有部分表皮鳞片向上翻起，出现局部脱落现象。另乳香、没药挥发油使角质层脂质的CH2伸缩振动峰和角质层角蛋白的酰胺伸缩振动峰均发生了相对的位移，表明，乳香、没药挥发油可能是使角质层中脂质及角蛋白的构象发生改变，增加角质层脂质双分子层的流动性，改变有序致密结构，来达到使皮肤的通透性增加，屏障作用降低的效果。由此可知，乳香没药可能通过增加药物在角质层分配，改变皮肤角质层结构来达到促渗效果。 　　[关键词] 乳香没药挥发油； 中药挥发油； 经皮给药； 促透机制； 角质层微观结构 　　Effects of Frankincense and Myrrh essential oil on transdermal 　　absorption of ferulic acid in Chuanxiong 　　GUAN Yongmei1，2， TAO Ling1， ZHU Xiaofang1， ZANG Zhenzhong1， JIN Chen1*， CHEN Lihua1 　　（1.Key Laboratory of Modern Preparation of Traditional Chinese Medicine under Ministry of Education， Jiangxi University of Traditional Chinese Medicine， Nanchang 330004， China； 　　2. Jiangxi Province Research Center of Extracts and Preparations Engineering Technology of 　　Traditional Chinese Medicine， Yichun 336000， China） 　　[Abstract] The aim of this paper was to explore the effects of Frankincense and Myrrh essential oil on transdermal absorption， and investigate the mechanism of permeation on the microstructure and molecular structure of stratum corneum. Through the determination of stratum corneum/medium partition coefficient of ferulicacid in Chuanxiong influenced by Frankincense and Myrrh essential oil， the effects of volatile oil of frankincense and Myrrh on the the microscopic and molecular structure of stratum corneum were explored by observation of skin stratum corneum structure under scanning electron microscopy， and investigation of frankincense and myrrh essential oil effects on the molecular structure of keratin and lipids in stratum corneum under Fourier transform infrared spectroscopy. The results showed that the oil could enhance the distribution of ferulic acid in the stratum corneum and medium， and to a certain extent damaged the imbricate structur